Splicing as target for identifying new active substances
OwnerPeter Schreier, Christian Aichingerinventors
Life of this patent
Estimating the term…
Abstract
The present invention relates to new deoxyribonucleic acid (DNA) constructs, to vectors and host organisms which contain these DNA constructs and which, owing to their use in specific methods, are suitable for indicating the loss of correct splicing, and to the use of these transgenic organisms or cells for identifying new active substances, and, finally, to methods of finding new active substances which are capable of preventing correct splicing and to the use of these methods in high-throughput screening (HTS) or ultra-high-throughput screening (UHTS).
Claims36
DNA construct comprising
- a) a promoter which is active in eukaryotes,
- b) a DNA sequence which has all elements of a functional intron, and
- c) a reporter gene,
- all three elements being functionally linked to each other.
Method for detecting the functionality of the splicing process in vivo.
Use of the splicing process in methods of identifying fungicidal, insecticidal and/or herbicidal compounds.
Use of splicing modulators as fungicides or antimycotics.
Use of splicing modulators as insecticides or herbicides.
Dependencies are inferred from the claim text.