Purified material having size and growth inhibitory effects on cells and tissues

A method of purifying a material (“Micrin”) with the ability to reduce cell, tissue and organ size, mass and growth, the method comprising collection of blood from a mammal and at least partially purifying said material, wherein during the collection a chelating agent is mixed with the collecting blood.

A method of making a material (“Micrin”) with the ability to reduce cell, tissue and organ size, mass and growth, the method comprising collecting blood from a mammal and harvesting plasma from it, purifying by molecular sizing, followed by ion exchange chromatography, wherein the elution process uses a salt solution of increasing concentration up to 1M, and selecting fractions that are active in an assay of apoptosis in vitro or an in vivo bioassay involving alterations in organ size.

A method of making a material (“Micrin”) with the ability to reduce cell, tissue and organ size, mass and growth, the method comprising purifying ovarian follicular fluid by molecular sizing, followed by ion exchange chromatography, wherein the elution process uses a salt solution of increasing concentration up to 1M, and selecting fractions that are active in an assay of apoptosis in vitro or an in vivo bioassay involving alterations in organ size.

Use of an antibody-to Micrin or another agent causing blockade of Micrin, for the manufacture of a medicament for preventing or treating a tissue-deficit disorder, or for controlling the shape, appearance or density of cells and tissues.