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AssigneeNorth Carolina State University
InventorsStefan Franzen, Simon E. Lappi
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Apparatus and method for acquiring an infrared spectrum of a sample having or suspected to have an amide I band, an amide II band, an amide III band, an amide A band, an OH stretching region or a combination thereof. A representative method includes providing a sample; providing an internal reflecting element (IRE) with a functionalized tip; contacting the sample with the IRE to form a sample-IRE interface; directing a beam of infrared (IR) radiation through the IRE under conditions such that the IR radiation interacts with the sample-IRE interface once; recording a reflectance profile over a range of preselected frequencies, whereby an infrared spectrum of the of a sample having or suspected of having an amide I band, an amide II band, an amide III band, an amide A band, an OH stretching region or a combination thereof, disposed in an aqueous solution is acquired. Representative apparatus includes an internal reflecting element (IRE) comprising a reflection face located on the IRE at a region of intended contact between the IRE and a solublized sample; an infrared radiation source for supplying an evanescent wave of infrared radiation and directing the same from the outside of the IRE to the inside thereof so as to cause the infrared radiation to be incident on the reflection face, wherein the infrared radiation is reflected from the reflection face once; a sample cell; a functionalized tip comprising a surface-immobilized probe that partially or completely fills the volume exposed to the evanescent wave; and a detector for detecting the once-reflected infrared radiation.
A solublized sample infrared spectrum measuring apparatus comprising:
A method of acquiring an infrared spectrum of a sample having or suspected to have an amide I band, an amide II band, an amide III band, an amide A band, an OH stretching region or a combination thereof, wherein the sample is disposed in an aqueous solution, the method comprising:
A method of generating a library of IR spectra of solublized samples in the frequency range of 50 to 3700 cm−1 by employing single pass ATR and a functionalized tip, the method comprising:
A single-pass FTIR-ATR method of identifying secondary structure present in one or more solublized samples having or suspected to have secondary structure, the method comprising:
A single pass FTIR-ATR method of identifying a degree of hydration of a first sample disposed in an aqueous solution, the method comprising:
A method of generating a calibration model for determining secondary structure in a test sample, the method comprising:
A method of detecting a binding event between a probe and a sample, the method comprising:
Dependencies are inferred from the claim text.