Quality control material for reagentless measurement of analytes

A method of monitoring calibration of a spectrophotometric apparatus comprising one or more calibration algorithms for a perflurocarbon-like blood substitute, turbidity, or a combination thereof, wherein said turbidity is measured in concentration units of a lipid emulsion, comprising:

• i) measuring absorbance of a quality control material with said apparatus to obtain a measurement, said quality control material exhibiting an absorbance spectra within the range from about 700 nm to about 1100 nm;
• ii) calculating one or more concentration values from said measurement using said one or more calibration algorithms;
• iii) comparing said one or more concentration values with an assigned value given to said quality control material for each of said perflurocarbon-like blood substitute, said turbidity, or a combination thereof, and
• iv) determining if there is a violation of a pre-established quality control rule, thereby monitoring said one or more calibration algorithms of said apparatus

A method of monitoring calibration of a spectrophotometric apparatus comprising one or more calibration algorithms for a perflurocarbon-like blood substitute, turbidity, or a combination thereof wherein said turbidity is measured in concentration units of a lipid emulsion, comprising:

• i) measuring absorbance of a quality control material with said apparatus to obtain a measurement, said quality control material exhibiting an absorbance spectra characterized as having a negative slope for a continuous spectral segment from about 5 nm to about 400 nm within the range of the absorbance spectra from about 700 nm to about 1100 nm;
• ii) calculating one or more concentration values from said measurement using said one or more calibration algorithms;
• iii) comparing said one or more concentration values with an assigned value given to said quality control material for of said one or more of a perflurocarbon-like blood substitute, turbidity, or a combination thereof, wherein said turbidity is measured in concentration units of a lipid emulsion;
• and
• iv) determining if there is a violation of a pre-established quality control rule, thereby monitoring said one or more calibration algorithms of said apparatus

A method for selecting one or more substances as a quality control material for monitoring at least one primary calibration algorithm on a spectrophotometric apparatus comprising:

• i) identifying a principal calibration wavelength for each of one or more analytes;
• ii) screening absorption spectra of said one or more substances;
• and
• iii) selecting one or more of said substances exhibiting a negative slope of said absorbance spectra, for a continuous spectral segment from about 5 nm to about 200 nm in length, said spectral segment including said principal calibration wavelength.

A method for selecting one or more substances as a quality control material for monitoring at least one primary calibration algorithm on a spectrophotometric apparatus for one or more of a perflurocarbon-like blood substitute and turbidity, wherein turbidity is measured in terms of concentration units of a lipid emulsion, comprising:

• i) identifying a principal calibration wavelength for each of one or more of of said perflurocarbon-like blood substitute and said turbidity;
• ii) screening absorption spectra of said one or more substances;
• and
• iii) selecting one or more of said substances exhibiting absorbance within the range from about 700 nm to about 1100 nm.

A method for selecting one or more substances as a quality control material for monitoring at least one primary calibration algorithm on a spectrophotometric apparatus for one or more of a perflurocarbon-like blood substitute and turbidity wherein turbidity is measured in terms of concentration units of a lipid emulsion, comprising:

• i) identifying a principal calibration wavelength for each of one or more of of said perflurocarbon-like blood substitute and said turbidity;
• ii) screening absorption spectra of said one or more substances;
• and
• iii) selecting one or more of said substances exhibiting absorbance spectra as having a negative slope for a continuous spectral segment from about 5 nm to about 400 nm within the range of wavelengths from about 700 nm to about 1100 nm.

A method of monitoring the calibration of a reagentless spectrophotometric apparatus comprising one or more calibration algorithms for one or more analytes in a sample, said method comprising:

• i) measuring absorbance of a quality control material with said reagentless spectrophotometric apparatus to obtain one or more measurements, said quality control material comprising one or more substances that absorb electromagnetic radiation, whereby predicted values for said one or more analytes can be obtained;
• ii) calculating one or more of said predicted values from said one or more measurements;
• iii) comparing said one or more of said predicted values with one or more assigned values given to said quality control material for said one or more analytes;
• and
• iv) determining if there is a violation of a pre-established quality control rule, thereby monitoring said calibration algorithms of said reagentless spectrophotometric apparatus.

A method for producing a corrected predicted value for an indicator of hemolysis in a sample, in the presence of met-Hb, said method comprising the steps of:

• i) developing a first primary calibration algorithm for one of a total Hb or an oxy-Hb, for predicting a first value for either said total Hb or said oxy-Hb in said sample;
• ii) deriving a second primary calibration algorithm for said met-Hb, for predicting a second value for said met-Hb in said sample;
• and
• iii) adding said predicted second value for said met-Hb to either said predicted first value for total Hb or said predicted first value for oxy-Hb, to a produce a said corrected predicted value for an indicator of hemolysis.

A method for flagging a predicted value for an indicator of hemolysis in a sample, in the presence of met-Hb, said method comprising the steps of:

• i) developing a first primary calibration algorithms for one of a total Hb or an oxy-Hb, for predicting a value for either said total Hb or said oxy-Hb in said sample;
• ii) deriving a second primary calibration algorithm for said met-Hb, for predicting a second value for said met-Hb in said sample;
• iii) determining if said predicted met-Hb value exceeds a pre-determined value;
• and
• iv) flagging said predicted value for said total Hb, said oxy-Hb or a combination thereof, when said predicted met-Hb value exceeds said pre-determined value.