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AssigneeThe Regents of the Universtiy of California
InventorsEric Verdin, Albert Jordan
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The present invention provides isolated cells that comprise, integrated into the genome of the cell, a transcription-competent immunodeficiency virus or a transcription-competent immunodeficiency virus-based retroviral vector. Under basal in vitro culture conditions, the immunodeficiency virus is latent, and the expression of the latent immunodeficiency virus can be reactivated. The invention further provides methods of making a subject cell. The invention further provides screening methods for identifying agents that activate a latent immunodeficiency virus; and screening method for identifying agents that block reactivation of latent immunodeficiency virus expression in response to T cell activation signals. The invention further provides agents identified in the subject screening assays. The invention further provides methods of treating an immunodeficiency virus infection.
An isolated cell that comprises, integrated into the genome of the cell, a recombinant transcription-competent immunodeficiency virus-based vector, wherein, under basal in vitro culture conditions, the immunodeficiency virus is latent, and wherein expression of the latent immunodeficiency virus can be reactivated.
A method of making an immortalized cell that comprises, integrated into the genome of the cell, a recombinant, transcription-competent human immunodeficiency virus (HIV) vector, wherein, under basal in vitro culture conditions, the HIV is latent, and wherein expression of the latent HIV can be reactivated, the method comprising:
An isolated immortalized cell that comprises, integrated into the genome of the cell, a recombinant transcription-competent human immunodeficiency virus (HIV) vector that comprises a nucleotide sequence encoding a selectable marker operably linked to a promoter, wherein, under basal in vitro culture conditions, the HIV is latent, and wherein expression of the latent HIV can be reactivated.
Dependencies are inferred from the claim text.