Phytase from bacillus subtilis, gene encoding said phytase, method for its production and use

A prokaryotic host cell transformed by a nucleic acid, wherein the nucleic acid is an isolated nucleic acid which encodes a phytase having a specific activity of at least about 20 U/mg protein and a pH optimum of at least 6.5,

• wherein said specific activity is determined by incubating said phytase in a solution containing about 100 mM Tris-HCl, at a pH of about 7.5, about 1 mM CaCl2, and about 1.6 mM sodium phytate at about 37° C. for about 30 minutes,
• wherein the isolated nucleic acid hybridizes to SEQ. ID. No. 1 under standard conditions either in 6×SSC, 0.6% SDS, 50° C. overnight for Southern blotting or for PCR:
• 5 MM Mg2++, Taq enzyme, premelting, 94° C. for 2 minutes and 30 cycles of melting at 92° C. for 20 seconds, annealing at 50° C. for 30 seconds and extension at 72° C. for 1 minute.

A eukaryotic host cell or organism transformed by a nucleic acid, wherein the nucleic acid is an isolated nucleic acid which encodes a phytase having a specific activity of at least about 20 U/mg protein and a pH optimum of at least 6.5,

• wherein said specific activity is determined by incubating said phytase in a solution containing about 100 mM Tris-HCl, at a pH of about 7.5, about 1 mM CaCl2, and about 1.6 mM sodium phytate at about 37° C. for about 30 minutes,
• wherein the isolated nucleic acid hybridizes to SEQ. ID. No. 1 under standard conditions either in 6×SSC, 0.6% SDS, 50° C. overnight for Southern blotting or for PCR:
• 5 mM Mg2++, Taq enzyme, premelting, 94° C. for 2 minutes and 30 cycles of melting at 92° C. for 20 seconds, annealing at 50° C. for 30 seconds and extension at 72° C. for 1 minute.

A method for the production of a nucleic acid which encodes a phytase, wherein a probe comprising a nucleic acid which encodes a phytase is hybridized to a sample suspected of containing said nucleic, under standard hydridization conditions either in 6×SSC, 0.6% SDS, 50° C. overnight or functional equivalents thereof for Southern blotting or for PCR 5 mM Mg2++, Taq enzyme, premelting, 94° C. for 2 minutes and 30 cycles of melting at 92° C. for 20 seconds, annealing at 50° C. for 30 seconds and extension at 72° C. for 1 minute,

• wherein the nucleic acid which encodes a phytase has a specific activity of at least about 20 U/mg protein and a pH optimum of at least 6.5, wherein said specific activity is determined by incubating said phytase in a solution containing about 100 mM Tris-HCl, at a pH of about 7.5, about 1 mM CaCl2, and about 1.6 mM sodium phytate at about 37° C. for about 30 minutes, wherein the isolated nucleic acid hybridizes to SEQ. ID. No. 1 under standard conditions either in 6×SSC, 0.6% SDS, 50° C. overnight for Southern blotting or for PCR:
• 5 mM Mg Taq enzyme, premelting, 94° C. for 2 minutes and 30 cycles of melting at 92° C. for 20 seconds, annealing at 50° C. for 30 seconds and extension at 72° C. for 1 minute.