Recombinant yeast for lignocellulose raw materials
AssigneeScandinavian Technology Group AB
InventorsRicardo Roman Cordero Otero, Barbel Hahn-Hagerdal, Willem Heber Van Zyl
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Abstract
The present invention relates to a method for obtaining a recombinant yeast of Saccharomyces cerevisiae, which ferments lignocellulose raw materials to ethanol, including introducing DNA into a yeast so as to cause the yeast to have introduced genes encoding xylose reductase, xylitol dehydrogenase and xylulokinase.
Claims36
A method for obtaining a recombinant yeast of Saccharomyces cerevisiae, which ferments lignocellulose raw materials to ethanol, including introducing DNA into a yeast so as to cause the yeast to have introduced genes encoding xylose reductase, xylitol dehydrogenase and xylulokinase.
A method as claimed in one or more of the preceeding claims wherein the lignocellulosic hydrolysate is a non-detoxified hydrolysate.
A method as claimed in one or more of the preceeding claims wherein the lignocellulosic hydrolysate is a detoxified hydrolysate.
A method as claimed in one or more of the preceeding claims wherein the lignocellulosic hydrolysate is a softwood derived hydrolysate.
A method as claimed in one or more of the preceeding claims wherein the lignocellulosic hydrolysate is a hardwood derived hydrolysate.
A method according one or more of the preceding claims, wherein the recombinant strain is maintained in continuous culture on xylose as carbon source at D=0.1 with Umax=0.14-0.15 h-1
A method according to one or more of the preceding claims, wherein the recombinant strain yields 0.4 g.g-1 biomass on xylose as carbon source at aerobic growth.
A method according to one or more of the preceding claims, wherein the recombiant strain simultanously utilises 20 g/l (all) and 15-16 g/l of xylose (4-5 g/l residual) in a continuous culture from a 20 g/l xylose plus 20 g/l of glucose feed
A recombinant yeast of Saccharomyces cerevisiae containing introduced genes encoding xylose reductase, xylitol dehydrogenase and xylulokinase.
Dependencies are inferred from the claim text.