Nitrilase from rhodococcus rhodochrous ncimb 11216
OwnerMarion Ress-Loschke, Bernhard Hauer +2inventors
InventorsMarion Ress-Loschke, Bernhard Hauer, Ralf Mattes, Dirk Engels
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Abstract
The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention additionally relates to an enzymatic process for preparing carboxylic acids from the corresponding nitrites.
Claims13
An isolated nucleic acid sequence which codes for a polypeptide having nitrilase activity, selected from the group of:
- a) a nucleic acid sequence having the sequence depicted in SEQ ID NO:
- 1,
- b) nucleic acid sequences which are derived from the nucleic acid sequence depicted in SEQ ID NO:
- 1 as a result of the degeneracy of the genetic code,
- c) derivatives of the nucleic acid sequence depicted in SEQ ID NO:
- 1, which code for polypeptides having the amino acid sequences depicted in SEQ ID NO:
- 2 and have at least 97% homology at the amino acid level, with negligible reduction in the enzymatic action of the polypeptides.
Dependencies are inferred from the claim text.