Capsaicin-decomposing/synthesizing enzymes and a method for producing the same
AssigneeOkayama University
InventorsKazuhiro Nakanishi, Takaharu Sakiyama, Koreyoshi Imamura
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Abstract
Capsaicin decomposing/synthesizing enzymes are disclosed, which each have the following physical and chemical properties: (1) function and substrate specificity: the enzyme catalyzes a decomposition and/or synthesis reaction of capsaicin and/or capsaicin analogs; (2) an optimal temperature range: near 55° C.; and(3) an optimal pH range of 7-8. Microorganisms to be used for producing these enzymes are also disclosed.
Claims17
A capsaicin decomposing/synthesizing enzyme having the following physical and chemical properties (1) to (3):
- (1) function and substrate specificity:
- the enzyme catalyzes a decomposition and/or synthesis reaction of capsaicin and/or capsaicin analogs;
- (2) an optimal temperature range:
- near 55° C.;
- and
- (3) an optimal pH range of 7-8.
A method for producing a capsaicin decomposing/synthesizing enzyme comprises the steps of incubating a microorganism belonging to the Streptomyces genus and being capable of producing the capsaicin decomposing/synthesizing enzyme, and collecting the capsaicin decomposing/synthesizing enzyme from a culture thereof.
A microorganism, which belong to a Streptomyces genus and is capable of producing a capsaicin and/or capsaicin analogs having the following physical and chemical properties:
- (1) function and substrate specificity:
- the enzyme catalyzes decomposition and/or synthesis reaction of capsaicin and/or capsaicin analogs;
- (2) an optimal temperature range:
- near 55° C.;
- and
- (3) an optimal pH range of 7-8.
A method for synthesizing capsaicin or a capsaicin analog comprises the step of reacting vanillylamine with a fatty acid in the presence of an enzyme having the following physical and chemical properties (1) to (3):
- (1) function and substrate specificity:
- the enzyme catalyzes a decomposition and/or synthesis reaction of capsaicin and/or capsaicin analogs;
- (2) an optimal temperature range:
- near 55° C.;
- and
- (3) an optimal pH range of 7-8.
Dependencies are inferred from the claim text.