Process for ex vitro sowing and germination of plant somatic embryos
AssigneeCellfor Inc.
InventorsDANIEL R. POLONENKO, ERIC EVERT VOOGT, POTTER ANN KATHRYN EASTMAN, SHIHE FAN
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AssigneeCellfor Inc.
InventorsDANIEL R. POLONENKO, ERIC EVERT VOOGT, POTTER ANN KATHRYN EASTMAN, SHIHE FAN
Estimating the term…
A multi-step process by which plant somatic embryos can be sown and germinated ex vitro using conventional seeding equipment, growing mixes, and plant propagation environments. The process most preferably comprises the steps of: placing a somatic embryo on or within a three-phase substrate, the phases comprising solid, liquid and gas phases, placing the substrate containing a somatic embryo into an environmentally-controlled plant-growing environment in which at least one environmental factor (i.e. moisture level within the three-phase substrate, atmospheric humidity, temperature, nutrients, ambient light intensity and diurnal photoperiod) may be controlled and manipulated, manipulating at least one of the environmental factors to enable and facilitate germination of the somatic embryo, and applying water and/or nutrient solutions at regular intervals, the intervals preferably ranging from 1 minute-24 hours, to the surface of the substrate in the form of microdroplets, preferably for a period of time ranging between 3 to eight weeks, such that somatic embryo imbibition, germination, growth and development occur. The process can be practiced in non-sterile conditions with “naked” fresh and/or HRHT-treated and/or desiccated embryos, i.e., non-encapsulated or otherwise uncoated embryos, and does not require the use of aseptic techniques or sterilized media or equipment.
A process for germinating somatic embryos having a period of somatic embryo germination, said process comprising the steps of:
The process according to claim I wherein the moisture content of the three-phase substrate is adjusted with a nutrient solution to a range of 60-85% prior to receiving a somatic embryo.
A process of germinating somatic embryos, which comprises:
A process for germinating somatic embryos having a period of somatic embryo germination, said process comprising the steps of:
Dependencies are inferred from the claim text.