Compositions and methods for recombinational cloning of nucleic acid molecules

A method for cloning or subcloning one or more desired nucleic acid molecules comprising

• (a) forming a combination by combining in vitro or in vivo
• (i) one or more Insert Donor molecules comprising one or more desired nucleic acid segments flanked by at least two recombination sites, wherein said recombination sites do not substantially recombine with each other;
• (ii) one or more Vector Donor molecules comprising at least two recombination sites, wherein said recombination sites do not substantially recombine with each other;
• (iii) an effective amount of at least one recombination protein;
• and
• (iv) all effective amount of at least one ribosomal protein;
• and
• (b) incubating said combination under conditions sufficient to transfer one or more of said desired segments into one or more of said Vector Donor molecules, thereby producing one or more desired Product nucleic acid molecules.

A method for cloning or subcloning desired nucleic acid molecules comprising

• a) forming a combination by combining in vitro or in vivo
• i) one or more Insert Donor molecules comprising one or more nucleic acid segments flanked by two or more recombination sites, wherein said recombination sites do not substantially recombine with each other;
• ii) two or more different Vector Donor molecules comprising two or more recombination sites, wherein said recombination sites do not substantially recombine with each other;
• iii) an effective amount of at least one recombination protein;
• and
• iv) an effective amount of at least one ribosomal protein;
• and
• b) incubating said combination under conditions sufficient to transfer one or more of said desired segments into said different Vector Donor molecules, thereby producing two or more different Product molecules.