Polypeptides having beta-1,3-galactosyl transferase activity
AssigneeNational Research Council of Canada
InventorsMichel Gilbert, Warren W. Wakarchuk
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Abstract
This invention provides prokaryotic glycosyltransferases, including a bifunctional sialyltransferase that has both an α2,3- and an α2,8-activity. A β1,4-GalNAc transferase and a β1,3-galactosyltransferase are also provided by the invention, as are other glycosyltransferases and enzymes involved in synthesis of lipooligosaccharide (LOS). The glycosyltransferases can be obtained from, for example, Campylobacter species, including C. jejuni. In additional embodiments, the invention provides nucleic acids that encode the glycosyltransferases, as well as expression vectors and host cells for expressing the glycosyltransferases.
Claims42
An isolated or recombinant nucleic acid molecule that comprises a polynucleotide sequence that encodes a polypeptide selected from the group consisting of:
- a) a polypeptide having lipid A biosynthesis acyltransferase activity, wherein the polypeptide comprises an amino acid sequence that is at least about 70% identical to an amino acid sequence encoded by nucleotides 350-1234 (ORF 2a) of the LOS biosynthesis locus of C. jejuni strain OH4384 as shown in SEQ ID NO:1;
- b) a polypeptide having glycosyltransferase activity, wherein the polypeptide comprises an amino acid sequence that is at least about 70% identical to an amino acid sequence encoded by nucleotides 1234-2487 (ORF 3a) of the LOS biosynthesis locus of C. jejuni strain OH4384 as shown in SEQ ID NO:1;
- c) a polypeptide having glycosyltransferase activity, wherein the polypeptide comprises an amino acid sequence that is at least about 50% identical to an amino acid sequence encoded by nucleotides 2786-3952 (ORF 4a) of the LOS biosynthesis locus of C. jejuni strain OH4384 as shown in SEQ ID NO:1 over a region at least about 100 amino acids in length;
- d) a polypeptide having β1,4-GalNAc transferase activity, wherein the GalNAc transferase polypeptide comprises an amino acid sequence that is at least about 77% identical to an amino acid sequence as set forth in SEQ ID NO:17 over a region at least about 50 amino acids in length;
- e) a polypeptide having β1,3-galactosyltransferase activity, wherein the galactosyltransferase polypeptide comprises an amino acid sequence that is at least about 75% identical to an amino acid sequence as set forth in SEQ ID NO:27 or SEQ ID NO:29 over a region at least about 50 amino acids in length;
- f) a polypeptide having α2,3 sialyltransferase activity, wherein the sialyltransferase polypeptide comprises an amino acid sequence that is at least about 66% identical over a region at least about 60 amino acids in length to an amino acid sequence as set forth in one or more of SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7 or SEQ ID NO:10;
- g) a polypeptide having sialic acid synthase activity, wherein the polypeptide comprises an amino acid sequence that is at least about 70% identical to an amino acid sequence encoded by nucleotides 6924-7961 of the LOS biosynthesis locus of C. jejuni strain OH4384 as shown in SEQ ID NO:1;
- h) a polypeptide having sialic acid biosynthesis activity, wherein the polypeptide comprises an amino acid sequence that is at least about 70% identical to an amino acid sequence encoded by nucleotides 8021-9076 of the LOS biosynthesis locus of C. jejuni strain OH4384 as shown in SEQ ID NO:1;
- i) a polypeptide having CMP-sialic acid synthetase activity, wherein the polypeptide comprises an amino acid sequence that is at least about 65% identical to an amino acid sequence encoded by nucleotides 9076-9738 of the LOS biosynthesis locus of C. jejuni strain OH4384 as shown in SEQ ID NO:1;
- j) a polypeptide having acetyltransferase activity, wherein the polypeptide comprises an amino acid sequence that is at least about 65% identical to an amino acid sequence encoded by nucleotides 9729-10559 of the LOS biosynthesis locus of C. jejuni strain OH4384 as shown in SEQ ID NO:1;
- and
- k) a polypeptide having glycosyltransferase activity, wherein the polypeptide comprises an amino acid sequence that is at least about 65% identical to an amino acid sequence encoded by a reverse complement of nucleotides 10557-11366 of the LOS biosynthesis locus of C. jejuni strain OH4384 as shown in SEQ ID NO:1.
An isolated or recombinantly produced polypeptide selected from the group consisting of:
- a) a polypeptide having lipid A biosynthesis acyltransferase activity, wherein the polypeptide comprises an amino acid sequence that is at least about 70% identical to an amino acid sequence encoded by nucleotides 350-1234 (ORF 2a) of the LOS biosynthesis locus of C. jejuni strain OH4384 as shown in SEQ ID NO:1;
- b) a polypeptide having glycosyltransferase activity, wherein the polypeptide comprises an amino acid sequence that is at least about 70% identical to an amino acid sequence encoded by nucleotides 1234-2487 (ORF 3a) of the LOS biosynthesis locus of C. jejuni strain OH4384 as shown in SEQ ID NO:1;
- c) a polypeptide having glycosyltransferase activity, wherein the polypeptide comprises an amino acid sequence that is at least about 50% identical to an amino acid sequence encoded by nucleotides 2786-3952 (ORF 4a) of the LOS biosynthesis locus of C. jejuni strain OH4384 as shown in SEQ ID NO:1 over a region at least about 100 amino acids in length;
- d) a polypeptide having β1,4-GalNAc transferase activity, wherein the GalNAc transferase polypeptide comprises an amino acid sequence that is at least about 77% identical to an amino acid sequence as set forth in SEQ ID NO:17 over a region at least about 50 amino acids in length;
- e) a polypeptide having β1,3-galactosyltransferase activity, wherein the galactosyltransferase polypeptide comprises an amino acid sequence that is at least about 75% identical to an amino acid sequence as set forth in SEQ ID NO:27 or SEQ ID NO:29 over a region at least about 50 amino acids in length;
- f) a polypeptide having α2,3 sialyltransferase activity, wherein the sialyltransferase polypeptide comprises an amino acid sequence that is at least about 66% identical to an amino acid sequence as set forth in SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7 or SEQ ID NO:10 over a region at least about 60 amino acids in length;
- g) a polypeptide having sialic acid synthase activity, wherein the polypeptide comprises an amino acid sequence that is at least about 70% identical to an amino acid sequence encoded by nucleotides 6924-7961 of the LOS biosynthesis locus of C. jejuni strain OH4384 as shown in SEQ ID NO:1;
- h) a polypeptide having sialic acid biosynthesis activity, wherein the polypeptide comprises an amino acid sequence that is at least about 70% identical to an amino acid sequence encoded by nucleotides 8021-9076 of the LOS biosynthesis locus of C. jejuni strain OH4384 as shown in SEQ ID NO:1;
- i) a polypeptide having CMP-sialic acid synthetase activity, wherein the polypeptide comprises an amino acid sequence that is at least about 65% identical to an amino acid sequence encoded by nucleotides 9076-9738 of the LOS biosynthesis locus of C. jejuni strain OH4384 as shown in SEQ ID NO:1;
- j) a polypeptide having acetyltransferase activity, wherein the polypeptide comprises an amino acid sequence that is at least about 65% identical to an amino acid sequence encoded by nucleotides 9729-10559 of the LOS biosynthesis locus of C. jejuni strain OH4384 as shown in SEQ ID NO:1;
- and
- k) a polypeptide having glycosyltransferase activity, wherein the polypeptide comprises an amino acid sequence that is at least about 65% identical to an amino acid sequence encoded by a reverse complement of nucleotides 10557-11366 of the LOS biosynthesis locus of C. jejuni strain OH4384 as shown in SEQ ID NO:1.
A reaction mixture for synthesis of a sialylated oligosaccharide, the reaction mixture comprising a sialyltransferase polypeptide which has both an α2,3 sialyltransferase activity and an α2,8 sialyltransferase activity, a galactosylated acceptor moiety, and a sialyl-nucleotide sugar;
- wherein the sialyltransferase transfers a first sialic acid residue from the sialyl-nucleotide sugar to the galactosylated acceptor moiety in an α2,3 linkage, and further transfers a second sialic acid residue to the first sialic acid residue in an α2,8 linkage.
A method for synthesizing a sialylated oligosaccharide, the method comprising incubating a reaction mixture that comprises a sialyltransferase polypeptide which has both an α2,3 sialyltransferase activity and an α2,8 sialyltransferase activity, a galactosylated acceptor moiety, and a sialyl-nucleotide sugar, under suitable conditions wherein the sialyltransferase polypeptide transfers a first sialic acid residue from the sialyl-nucleotide sugar to the galactosylated acceptor moiety in an α2,3 linkage, and further transfers a second sialic acid residue to the first sialic acid residue in an α2,8 linkage.
Dependencies are inferred from the claim text.