Biosynthesis of enediyne compounds by manipulation of C-1027 gene pathway
AssigneeWisconsin Alumni Research Foundation
InventorsBen Shen, Wen Liu
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Abstract
This invention provides nucleic acid sequences and characterization of the gene cluster responsible for the biosynthesis of the enediyne C-1027 (produced by Streptomyces globisporus). Methods are provided for the biosynthesis of enediynes, enediyne analogs and other biological molecules. This invention also provides enediyne and enediyne analogs biosynthesized by manipulation of the C-1027 gene pathway.
Claims20
A compound having the formula:
- 3
- wherein R1 is H or OH;
- R2 is Cl or H;
- R3 is OCH3 or H;
- with the proviso that R1 is not OH when R2 is Cl and R3 is OCH3.
An enediyne analogue produced by a transformed host cell comprising a C-1027 biosynthesis gene cluster sufficient to direct the assembly of a C-1027 enediyne analogue, wherein the gene cluster is no longer effective in producing naturally-occurring C-1027.
A compound having the formula:
- 4
- wherein R1 is H or OH;
- R2 is Cl or H;
- R3 is OCH3 or H;
- with the proviso that R1 is not OH when R2 is Cl and R3 is OCH3.
Dependencies are inferred from the claim text.