Antibiotic production (II)
OwnerHugo Cesar Gramajo, Eduardo Jose Rodriguezinventors
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Abstract
The nucleic acid and amino acid sequences of α1, α2, β and ε subunits of acetyl-CoA carboxylase (ACCase) from Streptomyces coelicolor are provided. This subunit structure differs from that of known acyl carboxylases. Materials and methods are provided of increasing ACCase activity and production of secondary metabolites (such as polyketides and especially antibiotics) by causing expression in Streptomyces of such nucleic acid. Also provided are methods of increasing ACCase activity and production of secondary metabolites (such as polyketides and especially antibiotics) by culturing Streptomyces in the presence of exogenous malonate.
Claims51
A nucleic acid comprising a nucleic acid sequence which encodes AccB polypeptide, or a nucleic acid sequence complementary thereto,
- wherein said AccB polypeptide has the amino acid sequence set out in FIG. 12A and/or the amino acid sequence encoded by the nucleic acid sequence set out in FIG. 12B.
A vector comprising the nucleic acid sequence set out in FIG. 12B under the control of the tipA promoter, whereby said vector is capable, after incorporation into Streptomyces coelicolor, of causing expression of AccB polypeptide having the amino acid sequence set out in FIG. 12A and/or the amino acid sequence encoded by the nucleic acid sequence set out in FIG. 12B.
A method of increasing acetyl-CoA carboxylase (ACCase) activity in a strain of Streptomyces coelicolor, the method comprising modifying said strain to express, or to increase expression of, nucleic acid encoding AccB polypeptide, said nucleic acid having the nucleic acid sequence set out in FIG. 12B.
A method of increasing production of a polyketide in Streptomyces coelicolor, the method comprising culturing said cells in the presence of exogenous malonate.
Dependencies are inferred from the claim text.