Subtilisin variants with improved characteristics
AssigneeDIREVO Bio Tech AG.
InventorsUlrich Kettling, Andre Koltermann, Oliver Kensch, Rene Kuhlemann, Ulrich Haupts, Markus Rarbach, Konrad Odendahl
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Abstract
The present invention provides subtilisin variants with improved characteristics, including improved substrate affinity, catalytic activity, catalytic efficiency and stability under washing conditions as well as overall wash performance. The subtilisin variants are therefore useful additives in cleaning compositions.
Claims61
A modified subtilisin which has at least one mutation in an amino acid sequence of a precursor subtilisin at a position numbered according to the amino acid sequence of the mature subtilisin BPN′ shown in SEQ ID NO:1, wherein said at least one mutation is selected from (a) E54D or E54G, (b) Q103R or Q103K, (c) T133K, (d) E156K or E156A, and (e) Y217H, or mutations at the respective positions which are homologous to said mutations (a) to (e), provided that in case of mutations E54D, Q103R, E156K or E156A the modified subtilisin has at least one more of said mutations (a) to (e).
A modified subtilisin comprising the following combination of mutations in an amino acid sequence of a precursor subtilisin at a position numbered according to the amino acid sequence of the mature subtilisin BPN′ shown in SEQ ID NO:1:
- Y6N/I31L/S38T/F50I/Q103R, P14T/Q103R/T130I/Q185H/K265N, and S145G/E156K/R247H.
Dependencies are inferred from the claim text.