Increasing production of proteins in gram-positive microorganisms
OwnerRobert M. Caldwell, Wilhelmus J. Quaxinventors
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Abstract
The present invention relates to secretion in Gram-positive microorganisms. The present invention provides the nuclei acid and amino acid sequences for the Bacillus subtilis secretion factor SecG. The present invention also provides means for increasing the secretion of heterologous or homologous proteins in Gram-positive microorganisms.
Claims27
A Gram-positive microorganism encoding a mutated Shine Delgarno sequence such that the translation of the transcript comprising secretion factor G (SecG) is modulated.
A Gram-positive microorganism encoding a mutated RNA polymerase sigma factor alpha (σA) sequence such that the expression of secretion factor G (SecG) is modulated.
An improved method for secreting a protein in a Gram-positive microorganism comprising the steps of obtaining a Gram-positive microorganism host cell comprising nucleic acid encoding SecG wherein said nucleic acid is under the control of expression signals capable of expressing SecG in a Gram-positive microorganism and further comprising nucleic acid encoding said protein;
- and culturing said microorganism under conditions suitable for expression of SecG and expression and secretion of said protein.
Dependencies are inferred from the claim text.