Aequorin as a growth marker in yeast
AssigneeSanofi-Aventis Deutschland GmbH
InventorsPauline Fraissignes, Denis Guedin
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Abstract
A yeast transduction pathway-inducible expression cassette for aequorin and yeast cells containing the expression cassette are disclosed, as well as methods of using the yeast cells in screening assays.
Claims16
A yeast cell comprising an aequorin-encoding deoxyribonucleic acid sequence expressibly linked to a promoter, wherein the promoter is responsive to activation of a transduction pathway.
An isolated deoxyribonucleic acid sequence comprising an aequorin encoding sequence expressibly linked to a promoter responsive to activation of a yeast transduction pathway.
A method for identifying compounds that modulate heterologous cell surface protein-mediated aequorin expression, the method comprising:
- (a) providing a yeast cell comprising an aequorin-encoding deoxyribonucleic acid sequence expressibly linked to a first, transduction pathway-activated, promoter and a heterologous cell surface protein-encoding deoxyribonucleic acid expressibly linked to a second promoter, wherein the heterologous cell surface protein is an element of the transduction pathway;
- (b) incubating the yeast cell with a compound;
- and
- (c) determining an amount of aequorin expression by the incubated yeast cell.
Dependencies are inferred from the claim text.