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AssigneeNational Research Council of Canada
InventorsMarianne M. Figueira, Louise Laramee, Denis Bourque, Louise Belanger, Denis Groleau, Lyne Morel, Mario Beland, Carlos B. Miguez
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The present invention relates to a method of producing a recombinant peptide, a recombinant protein, or a product from metabolic engineering using a genetically modified methylotrophic bacterium, and more particularly to Methylobacterium extorquens ATCC 55366. The method comprises introducing an expression vector into the methylotrophic bacterium, the expression vector comprising a polynucleotide sequence, encoding for a peptide or protein, or allowing for production of a product from metabolic engineering under the control of a regulated promoter. The method also comprises growing the genetically modified methylotrophic bacterium in a minimal salts medium lacking organic sugars and containing methanol. A metal ion may be used for regulating the expression of the polynucleotide sequence by the promoter.
A method of producing a recombinant peptide, a recombinant protein or a product from metabolic engineering using a genetically modified methylotrophic bacterium under the control of a regulated promoter from a methylotrophic microorganism of the same or different species as the methylotrophic bacterium, said method comprising the steps of:
An expression vector for producing a recombinant peptide, a recombinant protein or a product from metabolic engineering in a methylotrophic bacterium, wherein said expression vector comprises a polynucleotide sequence encoding for a peptide or a protein or allowing production of a product from metabolic engineering, under the control of a metal regulated promoter.
An expression vector for producing a recombinant peptide, a recombinant protein or a product from metabolic engineering in a methylotrophic bacterium grown in a minimal salts medium lacking organic sugars and containing methanol, wherein said expression vector comprises a polynucleotide sequence encoding for a peptide or a protein or allowing production of a product from metabolic engineering, under the control of a methylotrophic bacterium promoter.
Dependencies are inferred from the claim text.