Biosensor and related method
AssigneePurdue Research Foundation
InventorsRafael Gomez, Rashid Bashir, Arun K. Bhunia, Michael R. Ladisch, J. Paul Robinson
Life of this patent
Estimating the term…
Abstract
A method for collecting a microbiological substance utilizes a microfabricated biochip having a collection chamber. A fluid sample containing a microbiological entity of interest is delivered to the collection chamber in the biochip. Then a non-uniform electric field is generated in the collection chamber, to retain the microbiological entity of interest in the collection chamber. The microbiological entity is retained through dielectrophoresis induced by the energization of the electrodes by a periodically applied, alternating current.
Claims17
A method for collecting a microbiological substance, comprising:
- providing a microfabricated biochip having a collection chamber;
- delivering a fluid sample to said collection chamber in said biochip, said fluid sample containing a microbiological entity of interest;
- generating a non-uniform electric field in said collection chamber;
- and
- at least temporarily retaining said microbiological entity of interest in said collection chamber by virtue of said non-uniform electric field.
A biochip comprising a substrate microfabricated to include as integrated components:
- a collection chamber;
- a first channel segment extending to an inlet of said collection chamber;
- a second channel segment extending from an outlet of said chamber;
- and
- a retention structure for holding, in said chamber, carrier elements entraining a target microbiological species while permitting passage through said collection chamber of contaminant materials in a fluid stream, said retention structure including electrodes for generating a non-uniform electric field.
A method for collecting a microorganism, comprising:
- preparing a fluid sample containing at least one microorganism of a preselected type;
- delivering said fluid sample to a collection chamber having a volume less than approximately 1 microliter;
- and
- at least temporarily retaining said microorganism in said collection chamber by dielectrophoresis.
Dependencies are inferred from the claim text.