Method for evaluating matrix metalloprotease inhibitory activity
AssigneesShionogi & Co., Ltd., Fuji Photo Film Co., Ltd.
InventorsRyuji Maekawa, Takayuki Yoshioka, Rhoichi Nemori, Yutaka Tamura
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Abstract
A method for checking a matrix metalloproteinase-inhibitory activity present in a mammal that has received a compound exhibiting matrix metalloproteinase-inhibitory activity, which comprises utilizing in situ zymography, is provided.
Claims9
A method for evaluating a compound exhibiting matrix metalloproteinase-inhibitory activity for an in vivo matrix metalloproteinase-inhibitory activity displayed by the compound, which comprises subjecting to in situ zymography a specimen isolated from a mammal that has received the compound.
A method for evaluating a compound exhibiting matrix metalloproteinase-inhibitory activity for an in vivo matrix metalloproteinase-inhibitory activity displayed by the compound, which comprises;
- (1) administering to a mammal a compound exhibiting matrix metalloproteinase-inhibitory activity;
- (2) isolating a tissue or a fluid from the mammal to prepare a specimen;
- (3) contacting the isolated specimen onto a thin membrane comprising a substrate of matrix metalloproteinase;
- (4) incubating the specimen-thin membrane sample;
- (5) staining and then decolorizing the thin membrane;
- and
- (6) verifying the tone developed on the thin membrane.
A method for evaluating an anti-cancer agent exhibiting matrix metalloproteinase-inhibitory activity for its anti-cancer activity, which comprises subjecting to in situ zymography a specimen isolated from a mammal that has received the anti-cancer agent, and evaluating for an in vivo matrix metalloproteinase-inhibitory activity displayed by the agent as an indicator of the anti-cancer activity.
Dependencies are inferred from the claim text.