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US 2003/0157578 A1Published application
Aequorin as a growth marker in yeast
OwnerPauline Fraissignes, Denis Guedininventors
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Abstract
A yeast cell containing constitutively expressed aequorin and methods of using the cells in growth and toxicity assays are disclosed.
Claims5
A yeast cell comprising an aequorin-encoding deoxyribonucleic acid sequence expressibly linked to a constitutive yeast promoter.
A method of measuring the growth rate of a yeast cell culture, the method comprising:
- (a) providing a yeast cell comprising an aequorin-encoding deoxyribonucleic acid sequence expressibly linked to a constitutive yeast promoter,
- (b) incubating the yeast cell, and
- (c) measuring the amount of aequorin produced during the incubation.
A method for determining growth-regulating or toxic effects of a compound on a yeast cell culture, the method comprising:
- (a) providing a yeast cell comprising an aequorin-encoding deoxyribonucleic acid sequence expressibly linked to a constitutive yeast promoter,
- (b) incubating the yeast cell with the compound,
- (c) measuring the amount of aequorin produced during the incubation, and
- (d) comparing the measured amount of aequorin produced with that amount of aequorin produced by a similar yeast culture incubated in the absence of the compound.
1 more element
Dependencies are inferred from the claim text.