Nucleotide sequences coding for the MtrA and/or MtrB proteins
AssigneeDegussa AG
InventorsBrigitte Bathe, Natalie Schischka, Michael Bott, Steffen Schaffer
Life of this patent
Estimating the term…
Abstract
The present invention provides nucleotide sequences from Coryneform bacteria which code for the MtrA and/or MtrB proteins and processes for the fermentative preparation of amino acids using bacteria in which the mtrA and/or mtrB genes are attenuated.
Claims78
An isolated polynucleotide, which encodes a protein comprising the amino acid sequence of SEQ ID NO:2.
An isolated polynucleotide, which encodes a protein comprising the amino acid sequence of SEQ ID NO:3.
An isolated polynucleotide, which comprises SEQ ID NO:1.
A Coryneform bacterium, which comprises an attenuated mtrA gene.
A Coryneform bacterium, which comprises an attenuated mtrB gene.
Escherichia coli DSM 14227.
Escherichia coli DSM 14228.
A process for producing L-amino acids comprising culturing a bacterial cell in a medium suitable for producing L-amino acids, wherein said bacterial cell comprises an attenuated mtrA gene.
A process for producing L-amino acids comprising culturing a bacterial cell in a medium suitable for producing L-amino acids, wherein said bacterial cell comprises an attenuated mtrB gene.
An isolated polypeptide comprising the amino acid sequence of SEQ ID NO:2.
An isolated polypeptide, which has an amino acid sequence that is at least 90% identical to SEQ ID NO:2.
An isolated polypeptide comprising the amino acid sequence of SEQ ID NO:3.
An isolated polypeptide, which has an amino acid sequence that is at least 90% identical to SEQ ID NO:3.
An isolated polynucleotide consisting essentially of nucleotides 542 to 1219 of SEQ ID NO:1.
An isolated polynucleotide consisting essentially of nucleotides 1310-2818 of SEQ ID NO:1.
Dependencies are inferred from the claim text.