Novel methods of diagnosing breast cancer, compositions, and methods of screening for breast cancer modulators
AssigneeEos Biotechnology, Inc.
InventorsKurt C. Gish, David Mack
Life of this patent
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Abstract
Described herein are methods that can be used for diagnosis and prognosis of breast cancer. Also described herein are methods that can be used to screen candidate bioactive agents for the ability to modulate breast cancer. Additionally, methods and molecular targets (genes and their products) for therapeutic intervention in breast cancer are described.
Claims31
A method of screening drug candidates comprising:
- a) providing a cell that expresses an expression profile gene encoding BCX3 or fragment thereof;
- b) adding a drug candidate to said cell;
- and
- c) determining the effect of said drug candidate on the expression of said expression profile gene.
A method of screening for a bioactive agent capable of binding to BCX3 or a fragment thereof, said method comprising:
- a) combining said BCX3 or a fragment thereof and a candidate bioactive agent;
- and
- b) determining the binding of said candidate agent to said BCX3 or a fragment thereof.
A method for screening for a bioacfive agent capable of modulating the activity of BCX3, said method comprising:
- a) combining BCX3 and a candidate bioactive agent;
- and
- b) determining the effect of said candidate agent on the bioactivity of BCX3.
A method of evaluating the effect of a candidate breast cancer drug comprising:
- a) administering said drug to a patient;
- b) removing a cell sample from said patient;
- and
- c) determining the expression of a gene encoding BCX3 or fragment thereof.
A method of diagnosing breast cancer comprising:
- a) determining the expression of a gene encoding BCX3 or a fragment thereof in a first breast tissue of a first individual;
- and
- b) comparing said expression of said gene(s) from a second normal breast tissue from said first individual or a second unaffected individual;
- wherein a difference in said expression indicates that the first individual has breast cancer.
An antibody which specifically binds to BCX3 or a fragment thereof.
A method for screening for a bioactive agent capable of interfering with the binding of BCX3 or a fragment thereof and an antibody which binds to BCX3 or fragment thereof, said method comprising:
- a) combining BCX3 or fragment thereof, a candidate bioactive agent and an antibody which binds to BCX3 or fragment thereof;
- and
- b) determining the binding of BCX3 or fragment thereof and said antibody.
A method for inhibiting the activity of BCX3, said method comprising binding an inhibitor to BCX3.
A method of neutralizing the effect of BCX3 or a fragment thereof, comprising contacting an agent specific for said BCX3 or fragment thereof with said BCX3 or fragment thereof in an amount sufficient to effect neutralization.
A method of treating breast cancer comprising administering to a patient an inhibitor of BCX3.
A method for localizing a therapeutic moiety to breast cancer tissue comprising exposing said tissue to an antibody to BCX3 or fragment thereof conjugated to said therapeutic moiety.
A method of treating breast cancer comprising administering to an individual having said breast cancer an antibody to BCX3 or fragment thereof conjugated to a therapeutic moiety.
A method for inhibiting breast cancer in a cell, wherein said method comprises administering to a cell a composition comprising antisense molecules to a nucleic acid of FIG. 1.
A biochip comprising one or more nucleic acid segments encoding BCX3 or a fragment thereof, wherein said biochip comprises fewer than 1000 nucleic acid probes.
A method of eliciting an immune response in an individual, said method comprising administering to said individual a composition comprising BCX3 or a fragment thereof.
A method of eliciting an immune response in an individual, said method comprising administering to said individual a composition comprising a nucleic acid encoding BCX3 or a fragment thereof.
A method for determining the prognosis of an individual with breast cancer comprising determining the level of BCX3 in a sample, wherein a high level of BCX3 indicates a poor prognosis.
Dependencies are inferred from the claim text.