Human II type 5alpha-reductase promoter gene and uses thereof
OwnerJotaro Nakanishi, Toshihiko Hibinoinventors
Life of this patent
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Abstract
A DNA molecule containing the promoter gene region of the human type II 5α-reductase gene, its modifications, and a method for screening type II 5α-reductase transcription controllers by using the same.
Claims8
An isolated DNA molecule capable of acting as a promoter for the type II 5α-reductase gene derived from human beings, the DNA molecule being selected from the group consisting of:
- (a) a polynucleotide comprising the DNA sequence represented by SEQ ID NO:1, the polynucleotide comprising a continuous DNA sequence extending from position 1 to 6022;
- (b) a fragment of polynucleotide (a), the fragment comprising a continuous DNA sequence of at least about 50 nucleotides including the transcription starting point at position 5952 (T);
- and
- (c) a polynucleotide having the DNA sequence of polynucleotide (a) in which one or more nucleotides have been modified by substitution, deletion and/or addition, and containing a polynucleotide comprising a continuous DNA sequence of at least about 50 nucleotides including the transcription starting point at position 5952 (T).
A method for promoting or suppressing the expression of human type II 5α-reductase which comprises providing an expression vector containing an isolated DNA molecule encoding transcription factors for transcription factor binding motifs present in the DNA sequence represented by SEQ ID NO:1, and culturing a mammalian cell having the expression vector introduced thereinto.
Dependencies are inferred from the claim text.