Nucleic acid labeling by Thermoanaerobacter thermohydrosulfuricus DNA polymerase I variants
OwnerMaria Davis, Chockalingam Palaniappaninventors
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Abstract
An enzymatically active DNA polymerase or active fragment thereof, having at least 80% identity in its amino acid sequence to the DNA polymerase of Thermoanaerobacter thermohydrosulfuricus or fragment thereof, and having an amino acid alteration at position 720 in Tts Pol I or at position 426 in ΔTts Pol I or at a homologous position defined with respect to Tts DNA ploymerase I, having improved incorporation of nucleotide analogs and natural bases during DNA synthesis compared to unaltered enzyme.
Claims10
An enzymatically active DNA polymerase or active fragment thereof, having at least 80% identity in its amino acid sequence to the DNA polymerase of Thermoanaerobacter thermohydrosulfuricus or a fragment thereof, and having an amino acid alteration at position 720 in Tts Pol I or at position 426 in ΔTts Pol I or at a homologous position defined with respect to Tts DNA ploymerase I, having improved incorporation of nucleotide analogs and natural bases during DNA synthesis compared to unaltered enzyme.
Dependencies are inferred from the claim text.