Methods and reagents for peptide-BIR interaction screens
OwnerAlain Boudreault, Robert G. Korneluk +2inventors
InventorsAlain Boudreault, Robert G. Korneluk, Eric LaCasse, Peter Liston
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Abstract
The invention features a substantially pure polypeptide having a length of less than 100 amino acids and capable of forming a complex with a polypeptide that includes a BIR domain. The invention also features displacement assays in which the ability of a candidate compound to disrupt the interaction between a BIR domain-containing polypeptide and a polypeptide of the invention is indicative of the ability of the candidate compound to modulate IAP biological activity.
Claims20
A substantially pure polypeptide having a length of less than 100 amino acids and comprising a sequence selected from Seq ID NOs:
- 3-21, 26-43, 47-65, and 67-75, said polypeptide capable of forming a complex with a polypeptide comprising a BIR domain.
A method for identifying a compound that modulates IAP biological activity, said method comprising the steps of:
- a) contacting a first polypeptide having a length of less than 100 amino acids and comprising a sequence selected from Seq ID NOs:
- 3-21, 26-43, 47-65, and 67-75, and a second polypeptide comprising a BIR domain to form a complex between said first polypeptide and said second polypeptide;
- b) contacting said complex with a candidate compound;
- and
- c) measuring the displacement of said first polypeptide from said second polypeptide,
- wherein said displacement of said first polypeptide from said second polypeptide indicates that said candidate compound is a compound that modulates IAP biological activity.
A method for identifying a compound that modulates IAP biological activity, said method comprising the steps of:
- a) contacting a first polypeptide having a length of less than 100 amino acids and comprising a sequence selected from Seq ID NOs:
- 3-21, 26-43, 47-65, and 67-75 and a second polypeptide comprising a BIR domain in the presence of a candidate compound;
- and
- b) measuring binding of said first polypeptide and said second polypeptide, wherein a decrease in the amount of binding relative to the amount of binding of said first polypeptide and said second polypeptide in the absence of said candidate compound indicates that said candidate compound is a compound that modulates IAP biological activity.
Dependencies are inferred from the claim text.