US 2003/0157514 A1 · Polynucleotide encoding a novel pleckstrin homology domain and proline rich domain containing adapter protein, PMN29

Abstract

The present invention provides novel polynucleotides encoding PMN29 polypeptides, fragments and homologues thereof. Also provided are vectors, host cells, antibodies, and recombinant and synthetic methods for producing said polypeptides. The invention further relates to diagnostic and therapeutic methods for applying these novel PMN29 polypeptides to the diagnosis, treatment, and/or prevention of various diseases and/or disorders related to these polypeptides. The invention further relates to screening methods for identifying agonists and antagonists of the polynucleotides and polypeptides of the present invention.

An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence selected from the group consisting of:

(a) a polynucleotide fragment of SEQ ID NO:1 or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:1;
(b) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:2 or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:1;
(c) a polynucleotide encoding a polypeptide domain of SEQ ID NO:2 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:1;
(d) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:2 or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:1;
(e) a polynucleotide encoding a polypeptide of SEQ ID NO:2 or the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:1, having cell proliferation or apoptosis modulatory activity;
(f) an isolated polynucleotide comprising nucleotides 70 to 1539 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 490 of SEQ ID NO:2 minus the start codon;
(g) an isolated polynucleotide comprising nucleotides 73 to 1539 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 490 of SEQ ID NO:2 including the start codon;
(h) an isolated polynucleotide comprising nucleotides 70 to 1041 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 324 of SEQ ID NO:2 including the start codon;
(i) an isolated polynucleotide comprising nucleotides 73 to 1041 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 324 of SEQ ID NO:2 minus the start codon;
(j) an isolated polynucleotide comprising nucleotides 70 to 720 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 217 of SEQ ID NO:2 including the start codon;
(k) an isolated polynucleotide comprising nucleotides 73 to 720 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 217 of SEQ ID NO:2 minus the start codon;
(l) an isolated polynucleotide comprising nucleotides 70 to 414 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 115 of SEQ ID NO:2 including the start codon;
(m) an isolated polynucleotide comprising nucleotides 73 to 414 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 115 of SEQ ID NO:2 minus the start codon;
(n) an isolated polynucleotide comprising nucleotides 129 to 426 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide corresponding to amino acids 19 to 119 of SEQ ID NO:2;
(o) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:1;
and
(p) a polynucleotide capable of hybridizing under stringent conditions to any one of the polynucleotides specified in (a)-(o), wherein said polynucleotide does not hybridize under stringent conditions to a nucleic acid molecule having a nucleotide sequence of only A residues or of only T residues.

19 more elements

Polynucleotide encoding a novel pleckstrin homology domain and proline rich domain containing adapter protein, PMN29

Life of this patent

Polynucleotide encoding a novel pleckstrin homology domain and proline rich domain containing adapter protein, PMN29

Life of this patent

Abstract

Claims19