Tramdorins and methods of using tramdorins
AssigneeMcLaughlin Research Institute
InventorJohn R. Bermingham JR.
Life of this patent
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Abstract
A family of transmembrane domain proteins, the tramdorins, has been identified in human, mouse and rat. A variety of uses for these proteins is contemplated, including but not limited to peripheral myclination, the etiology of parathyroid adenomas, and the diagnosis and treatment of Central Nervous System (CNS) and non-CNS disorders.
Claims9
An isolated nucleic acid sequence selected from the group consisting of the cDNA sequence encoding mouse tramd 2 (SEQ ID NO:
- 1), the cDNA sequence encoding mouse tramd 3 (SEQ ID NO:
- 2), the cDNA sequence encoding human tramd 1 (SEQ ID NO:
- 3), the cDNA sequence encoding human tramd 2 (SEQ ID NO:
- 4), the cDNA sequence encoding human tramd 3 (SEQ ID NO:
- 5), the cDNA sequence encoding rat tramd 1 (SEQ ID NO:
- 6), the genomic sequence of mouse tramd 1 (SEQ ID NO:
- 7) and the genomic sequence of mouse tramd 3 (SEQ ID NO:
- 8).
A method of detecting a tramdL-STK4/Mrs-1/Krs-2 fusion in isolated human parathyroid adenomas comprising:
- (a) providing:
- (i) isolated tissue or cultured cells derived from human parathyroid adenomas, (ii) differentially labeled tramdL and STK4/Mrs-1/Krs-2 probes;
- (b) hybridizing said probes to said tissue, and;
- (c) detecting the hybridization pattern of said differentially labeled probes.
A method of identifying proteins which interact with mouse tramd 1, comprising
- (a) providing:
- (i) a first recombinant vector comprising a portion of mouse tramd 1 in operable combination with the DNA binding domain of a transcriptional activator, such that a chimeric protein will be expressed, (ii) a population of second recombinant vectors, wherein said population comprises a library of cDNA sequences in operable combination with the activation domain of a transcriptional activator, such that a population of chimeric proteins will be expressed, and (iii) a yeast host comprising a reporter gene in operable combination with the DNA binding sites for said transcriptional activator;
- (b) introducing said first vector and said population of second vectors into said yeast host to generate a population of transformed yeast;
- (c) subjecting said population of transformed to yeast to conditions such that said chimeric proteins are expressed;
- (d) screening said population for members which express said reporter gene, and;
- (e) isolating the coda fragment from said second vector from members of the population which express the reporter gene.
Dependencies are inferred from the claim text.