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US 2003/0157503 A1Published application
Compositions and methods for performing biological reactions
OwnerMark W McGarry, Travis Johnson +1inventors
InventorsMark W McGarry, Travis Johnson, George W Hawkins
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Abstract
The present invention relates to an apparatus for performing biological reactions. Specifically, the invention relates to an apparatus for performing nucleic acid hybridization reactions on a substrate layer having a multiplicity of oligonucleotide binding sites disposed thereon, using substrates with flexible covers.
Claims11
An apparatus for performing biological reactions comprising:
- a) a substrate having a first surface and a second surface;
- b) an array of biomolecules positioned on said first surface;
- c) a porous flexible layer affixed to said first surface by an adhesive layer to create a reaction volume;
- d) a gas diffusion accelerator;
- and
- e) a first port extended from said second surface to said reaction volume of said first surface.
3 more elements
A method of detecting the presence of a target analyte in a sample comprising:
- a) contacting said sample with an apparatus comprising:
- i) a substrate having a first surface and a second surface;
- ii) an array of biomolecules positioned on said first surface;
- iii) a porous flexible layer affixed to said first surface by an adhesive layer to create a reaction volume;
- iv) a gas diffusion accelerator;
- and
- v) a first port extended from said second surface to said reaction volume of said first surface;
- under conditions whereby said target analyte will bind to at least one of said biomolecules;
- b) utilizing said gas diffusion accelerator to remove gas bubbles;
- and
- c) detecting the presence of said target analyte.
8 more elements
Dependencies are inferred from the claim text.