Analysing polynucleotide sequences
AssigneeOxford Gene Technology Limited
InventorEdwin Southern
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Abstract
This invention provides an apparatus and method for analyzing a polynucleotide sequence, either an unknown sequence or a known sequence. A support carries an array of the whole or a chosen part of a complete set of oligonucleotides, which are capable of taking part in hybridization reactions. The polynucleotide sequence, or fragments thereof, are labeled and applied to the array under hybridizing conditions. Applications include analysis of known point mutations, genomic fingerprinting, linkage analysis, characterization of mRNAs, mRNA populations, and sequence determination.
Claims16
Apparatus for analyzing a polynucleotide sequence, comprising a support and attached to a surface thereof an array of the whole or a chosen part of a complete set of oligonucleotides of chosen lengths, the different oligonucleotides occupying separate cells of the array and being capable of taking part in hybridization reactions.
Apparatus for studying differences between polynucleotide sequences, comprising a support and attached to a surface thereof an array of the whole or a chosen part of a complete set of oligonucleotides of chosen lengths comprising the polynucleotide sequences, the different oligonucleotides occupying separate cells of the array and being capable of taking part in hybridization reactions.
A method of analyzing a polynucleotide sequence, by the use of a support to the surface of which is attached an array of the whole or a chosen part of a complete set of oligonucleotides of chosen lengths, the different oligonucleotides occupying separate cells of the array, which method comprises labeling the polynucleotide sequence or fragments thereof to form labeled material,
- applying the labeled material under hybridization conditions to the array, and observing the location of the label on the surface associated with particular members of the set of oligonucleotides
Dependencies are inferred from the claim text.