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US 2003/0157492 A1Published application
Method to isolate DNA
AssigneeQiegen North American Holdings, Inc
InventorsEllen M. Heath, Nathaniel W. Morken, Kristen Campbell Benedict
Life of this patent
Estimating the term…
Abstract
An improved method for isolating DNA from biological samples is provided.
Claims65
A method for isolating DNA from a biological sample comprising the following sequential steps:
- (a) separating the biological material comprising DNA from the remainder of the biological sample;
- (b) contacting the separated biological material comprising DNA of step (a) with a hypertonic, high salt reagent so as to form a suspension of said biological material containing DNA;
- (c) contacting the suspension of step (b) with a lysis reagent to form a lysate comprising DNA and non-DNA biological components released from the biological material;
- and
- (d) physically separating the DNA from the non-DNA biological components in the lysate of step (c) to yield isolated DNA.
2 more elements
A method for isolating DNA from a biological sample comprising biological material comprising DNA comprising the following sequential steps:
- (a) contacting the biological material comprising DNA with a hypertonic, high salt reagent so as to form a suspension of the biological material comprising DN;
- (b) contacting the suspension of step (b) with a lysis reagent to form a lysate comprising DNA and non-DNA biological components released from the biological material;
- and
- (c) physically separating the DNA from the non-DNA biological components in the lysate of step (c) to yield isolated DNA.
1 more element
A method for isolating DNA from a biological sample comprising cells comprising the following sequential steps:
- (a) separating the cells comprising DNA from the remainder of the biological sample;
- (b) contacting the separated cells comprising DNA of step (a) with a hypertonic, high salt reagent so as to form a suspension of said biological cells;
- (c) contacting the suspension of step (b) with a lysis reagent to form a lysate comprising DNA and non-DNA biological components of the biological material;
- and
- (d) physically separating the DNA from the non-DNA biological components of the lysate of step (c) to yield isolated DNA.
2 more elements
A method for isolating DNA from a whole blood sample comprising red blood cells and white blood cells comprising the following sequential steps:
- (a) contacting the biological sample with a red blood lysis reagent to lyse the red blood cells;
- (b) separating the white blood cells from the lysed red blood cells;
- (c) contacting the white blood cells with a hypertonic, high-salt reagent to suspend the white blood cells in a solution of said hypertonic, high-salt reagent;
- (d) subsequently contacting the white blood cells of step (c) with a lysis reagent to form a lysate containing DNA and non-DNA cellular material;
- and
- (e) physically separating the DNA from non-DNA cellular material of the lysate to yield isolated DNA.
3 more elements
Dependencies are inferred from the claim text.