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US 2003/0157478 A1Published application
Method and device for the rapid clinical diagnosis of hepatitis B virus (HBV) infection in biological samples
AssigneeReliance Life Sciences Private Limited
InventorsVijay Sharma, Venkata Ramana Kondiboyina
Life of this patent
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Abstract
There is provided a method and kit for rapid clinical diagnosis of HBV in which the amplimers are transcripts of a pre-core or envelop region gene of HBV. The amplicons are hybridized to a specific oligonucleotide probe, which allows the amplicons to be detected.
Claims35
A kit for amplifying HBV nucleic acid comprising:
- a first amplification primer having the sequence:
- 5′-atactgcggaactcctagc-3′ [SEQ. ID. NO. 1]
- and a second amplification primer having the sequence:
- 5′-gttcacggtggtctccatgcgacgtgc-3′ [SEQ. ID. NO. 2]
1 more element
A kit for detecting an HBV nucleic acid comprising an oligonucleotide probe having the sequence:
- 5′-gggcgcacctctctttacgcgg-3′ [SEQ. ID. NO. 3]
A method for detecting HBV nucleic acid in a biological sample comprising the steps of:
- extracting HBV nucleic acid a biological sample;
- amplifying the HBV nucleic acid using a first primer having the sequence
- 5′-atactgcggaactcctagc-3′ [SEQ. ID. NO. 1]
- and a second primer having the sequence
- 5′-gttcacggtggtctccatgcgacgtgc-3′ [SEQ. ID. NO. 2]
- and detecting the HBV nucleic acid using an oligonucleotide probe having the sequence:
- 5′-gggcgcacctctctttacgcgg-3′ [SEQ. ID. NO. 3]
4 more elements
Dependencies are inferred from the claim text.