Immortalized human keratinocyte cell line
AssigneeWisconsin Alumni Research Foundation
InventorsB. Lynn Allen-Hoffmann, Sandra J. Schlosser, Michael A. Pickart
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Abstract
A spontaneously immortalized human keratinocyte cell line is disclosed. In a preferred embodiment, this cell line is ATCC 12191. In another embodiment of the invention, a method of assaying the effect of a test tumor cell modulation agent is disclosed. The method comprises the steps of obtaining a human stratified squamous epithelial cell culture, wherein the culture comprises human malignant squamous epithelial cells and spontaneously immortalized human keratinocytes, wherein the culture forms a reconstituted epidermis. One then treats the epidermis with a test tumor cell modulation agent and evaluates the growth of the malignant cells within the epidermis.
Claims24
An immortalized human kerotinocyte cell line, wherein the cell line comprises a normal chromosomal complement of 46 with the exception of an extra isochromosome of the long arm of chromosome 8.
A method of assaying the effect of a test tumor cell modulation agent comprising the steps of
- a) obtaining a human stratified squamous epithelia cell culture, wherein the culture comprises malignant squamous epithelia cells and spontaneously immortalized human keratinocytes and wherein this culture forms a reconstructed epidermis,
- b) treating the epidermis with a test tumor cell therapeutic agent, and
- c) evaluating the malignant cells within the epidermis.
An organotypic culture comprising a combination of immortalized human keratinocytes and human squamous cell carcinomas (SCC) cells.
Dependencies are inferred from the claim text.