Compositions comprising reproductive cell media and methods for using such compositions
AssigneeMinitube of America, Inc.
InventorsKevin J. Rozeboom, Mark E. Wilson
Life of this patent
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Abstract
Disclosed are compositions for mammalian, avian or piscian reproductive cells and methods for the collection, holding, processing, in vitro fertilization, sexing culturing, or storing (including long-term cryopreservation) of mammalian, avian, or piscian reproductive sperm cells. The compositions comprise a suitable reproductive cell media and a transforming growth factor, an insulin-like growth factor, or zinc, and, optionally, inositol, transferrin, or fructose, or combinations thereof.
Claims53
A composition comprising a sperm cell medium for mammalian, avian, or piscian sperm cells, wherein the medium comprises at least one growth factor selected from the group consisting of insulin-like growth factors and transforming growth factors.
A composition comprising a reproductive cell medium for mammalian, avian, or piscian reproductive cells, wherein the medium comprises zinc.
A composition comprising a sperm cell medium for mammalian, avian, or piscian sperm cells, wherein the medium comprises transferrin.
A composition comprising a reproductive cell medium for mammalian, avian, or piscian reproductive cells, wherein the medium comprises a growth factor selected from the group consisting of activated TGFβ-1, activated TGFβ-2, and activated IGF-1.
A composition comprising a reproductive cell medium for mammalian, avian, or piscian reproductive cells, wherein the medium comprises inositol, and wherein the inositol is present in a concentration of from about 1 mg/L to about 1 g/L.
A composition comprising a sperm cell medium for porcine sperm cells, wherein the medium comprises TGFβ-1, TGFβ-2, and IGF-1
A composition comprising sperm cell medium for porcine sperm cells, wherein the medium comprises activated TGFβ-1, activated TGFβ-2, and activated IGF-1.
Dependencies are inferred from the claim text.