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US 2003/0157230 A1Published application
Process for stabilizing proteins in an acidic environment with a high-ester pectin
AssigneeDanisco A/S
InventorsTove Martel Ida Elsa Christensen, Jette Dina Kreiberg, Hanne Thorsoe, Hans Christian Buchholt, Preben Rasmussen, John Nielsen
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Abstract
A process is described wherein there is added to an acidic environment, which contains at least one protein, a block-wise enzymatically de-esterified pectin, and wherein the pectin is a high ester pectin. Also described is a recombinant pectin methyl esterase.
Claims19
A process comprising:
- a) purifying a pectin methyl esterase (PME) enzyme capable of block-wise enzymatically de-esterifying pectin;
- b) adding said purified pectin methyl esterase (PME) enzyme capable of block-wise enzymatically de-esterifying pectin to a pectin;
- c) preparing a block-wise enzymatically de-esterified pectin from said pectin by said purified pectin methyl esterase (PME) enzyme capable of block-wise enzymatically de-esterifying pectin;
- i) wherein said block-wise enzymatically de-esterified pectin is a high ester pectin;
- and
- ii) wherein said block-wise enzymatically de-esterified pectin contains from about 70% to about 80% ester groups, preferably about 76% ester groups;
- d) adding said block-wise enzymatically de-esterified pectin to an acidic environment comprising at least one protein;
- and
- e) stabilising said protein by said block-wise enzymatically de-esterified pectin.
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