Modified nodavirus rna for gene delivery
AssigneeChiron Corporation
InventorsGiuliano Bensi, Alessio Zippo
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Abstract
The invention provides a nodavirus RNA1 molecule modified to include a heterologous insertion which is downstream of its replicase ORF and, preferably, its B2 ORF. The insertion preferably comprises one or more protein-coding regions. The modified RNA1 may be packaged in a VLP, such as a papillomavirus VLP. The small size of nodavirus RNA1 makes it ideal for HPV packaging.
Claims29
A modified nodavirus RNA1 molecule which includes a heterologous insertion downstream of the replicase ORF of said RNA1.
The RNA1 molecule of any preceding claim, wherein the heterologous insertion is more than 5 nucleotides upstream of 3′ end of RNA1.
The RNA1 molecule of any preceding claim, wherein the heterologous insertion comprises a protein-coding region.
The RNA1 molecule of any preceding claim, wherein RNA1 comprises a sequence that can specifically interact with a protein sequence.
A VLP containing the RNA1 molecule of any preceding claim.
The use of the tat/TAR interaction for packaging nucleic acids within a VLP.
RNA comprising a RNA1 sequence from a nodavirus, characterised in that the RNA1 sequence contains a heterologous insertion downstream of its replicase ORF.
Dependencies are inferred from the claim text.