Chlamydia antigens and corresponding DNA fragments and uses thereof
AssigneeAventis Pasteur Limited
InventorsAndrew D. Murdin, Raymond P. Oomen, Joe Wang, Pamela Dunn
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Abstract
The present invention provides a method of nucleic acid, including DNA, immunization of a host, including humans, against disease caused by infection by a strain of Chlamydia, specifically C. pneumoniae, employing a vector containing a nucleotide sequence encoding a 98 kDa outer membrane protein of a strain of Chlamydia pneumoniae and a promoter to effect expression of the 98 kDa outer membrane protein gene in the host. Modifications are possible within the scope of this invention.
Claims31
A nucleic acid molecule comprising a nucleic acid sequence which encodes a polypeptide selected from any of:
- (a) SEQ ID Nos:
- 3 and 4;
- (b) an immunogenic fragment comprising at least 12 consecutive amino acids from a polypeptide of (a);
- and
- (c) a polypeptide of (a) or (b) which has been modified to improve its immunogenicity, wherein said modified polypeptide is at least 75% identical in amino acid sequence to the corresponding polypeptide of (a) or (b).
A nucleic acid molecule comprising a nucleic acid sequence selected from any of:
- (a) SEQ ID Nos:
- 1 and 2;
- (b) a sequence which encodes a polypeptide encoded by SEQ ID No:
- 1 or 2;
- (c) a sequence comprising at least 38 consecutive nucleotides from any one of the nucleic acid sequences of (a) and (b);
- and
- (d) a sequence which encodes a polypeptide which is at least 75% identical in amino acid sequence to the polypeptides encoded by SEQ ID No:
- 1 or 2.
A nucleic acid probe of 5 to 100 nucleotides which hybridizes under stringent conditions to the nucleic acid molecule of SEQ ID No:
- 1 or 2, or to a homolog or complementary or anti-sense sequence of said nucleic acid molecule.
A primer of 10 to 40 nucleotides which hybridizes under stringent conditions to the nucleic acid molecules of SEQ ID No:
- 1 or 2, or to a homolog or complementary or anti-sense sequence of said nucleic acid molecule.
A polypeptide comprising an amino acid sequence selected from any of:
- (a) SEQ ID Nos:
- 3 and 4;
- (b) an immunogenic fragment comprising at least 12 consecutive amino acids from a polypeptide of (a);
- and
- (c) a polypeptide of (a) or (b) which has been modified to improve its immunogenicity, wherein said modified polypeptide is at least 75% identical in amino acid sequence to the corresponding polypeptide of (a) or (b).
Expression plasmid pCAI640.
Dependencies are inferred from the claim text.