Tumour-specific animal proteins
OwnerTeresa Elisa Virginia Cabezon-Silva, Jean-Pol Cassart +3inventors
InventorsTeresa Elisa Virginia Cabezon-Silva, Jean-Pol Cassart, Thierry Coche, Swann Romain Jean-Thomas Gaulis, Carlota Vinals Y De Bassols
Life of this patent
Estimating the term…
Abstract
CASB7439 polypeptides and polynucleotides, immunogenic compositions comprising them and methods for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilizing CASB7439 polypeptides and polynucleotides in diagnostics, and vaccines for prophylactic and therapeutic treatment of cancers, particularly colorectal cancers, autoimmune diseases, and related conditions.
Claims33
An immunogenic composition comprising an isolated polypeptide wherein the polypeptide comprises a member selected from the group consisting of
- (a) an amino acid sequence which has at least 90% identity to SEQ ID NO:2;
- (b) an immunogenic fragment of the amino acid sequence of (a), wherein the immunogenic fragment is at least 90% identical to an aligned contiguous segment of SEQ ID NO:2;
- and
- (c) an immunogenic fragment of the amino acid sequence of (a) that matches an aligned contiguous segment of SEQ ID NO:2 with no more than five single amino acid substitutions, deletions or additions,
- wherein the composition, when administered to a subject, induces an immune response that recognizes a polypeptide having the sequence of SEQ ID NO:2.
A polypeptide comprising the amino acid sequence set forth in SEQ ID NO:10.
An immunogenic composition comprising an isolated polypeptide encoded by an isolated first polynucleotide wherein the isolated first polynucleotide hybridizes under stringent conditions to a second polynucleotide which encodes the polypeptide of SEQ ID NO:2;
- wherein stringent conditions comprise overnight incubation at 42° C. in a solution comprising:
- 50% formamide, 5×SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH7.6), 5×Denhardt's solution, 10% dextran sulfate, and 20 micrograms/ml denatured, sheared salmon sperm DNA, followed by washing the filters in 0.1×SSC at about 65° C.;
- wherein the composition, when administered to a subject, induces an immune response that recognizes a polypeptide having the sequence of SEQ ID NO:2.
An isolated polynucleotide segment comprising a polynucleotide sequence or the full complement of the entire length of the polynucleotide sequence, wherein the polynucleotide sequence is identical to the sequence of nucleotides 545 to 1126 of SEQ ID NO:1, except that, over the entire length corresponding to nucleotides 545 to 1126 of SEQ ID NO:1, nn nucleotides are substituted, inserted or deleted, wherein nn satisfies the following expression
- nn≦xn−(xn·y)
- wherein xn is the total number of nucleotides corresponding to nucleotides 545 to 1126 of SEQ ID NO:1, y is at least 0.90, and wherein any non-integer product of xn and y is rounded down to the nearest integer before subtracting the product from xn;
- and wherein the polynucleotide sequence detects a polynucleotide of SEQ ID NO:1.
Dependencies are inferred from the claim text.