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US 2003/0157091 A1Published application
Multi-functional proteins
AssigneeDyax Corp.
InventorHenricus Renerus Jacobus Mattheus Hoogenboom
Life of this patent
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Abstract
Disclosed are compositions and methods to generate functional target-binding proteins from at least two separate polypeptide chains, one including the target-binding domain, the other including an effector domain. For example, the two separate chains are reconstituted as a functional protein by a non-covalent binding interaction mediated by an interaction sequence or by intein-mediated ligation.
Claims20
A protein comprising:
- a first polypeptide that includes a first immunoglobulin domain and a first interaction sequence, wherein the first interaction sequence specifically recognizes a second interaction sequence;
- and
- a second polypeptide that includes the second interaction sequence and an effector domain that does not include an immunoglobulin variable domain.
A compound comprising:
- a first polypeptide that includes at least a part of a first target-binding domain and a first interaction sequence;
- and
- a second polypeptide that includes the second interaction sequence and at least a part of an effector domain;
- wherein the first interaction sequence can bind to the second interaction sequence and the effector domain is has one or more of the following properties:
- a) binds (e.g., specifically binds) to a surface of a cell, b) is functional in an extracellular environment, or c) is a detectable label (i.e., other than being antigenic).
2 more elements
A method comprising:
- providing (i) a first cell that includes a first nucleic acid that encodes a first polypeptide that includes a first immunoglobulin domain and a first interaction sequence, and (ii) a second cell that includes a second nucleic acid encoding a second polypeptide that includes a second interaction sequence and an effector domain;
- culturing the first cell under conditions such that the first polypeptide is expressed and the second cell under conditions such that the second polypeptide is expressed;
- isolating the first polypeptide from the first cell and the second polypeptide from the second cell;
- and
- contacting the first polypeptide to the second polypeptide to form a complex.
2 more elements
Dependencies are inferred from the claim text.