Stabilizing peptides, polypeptides and antibodies which include them
OwnerEugeno Benvenuto, Rosella Franconi +2inventors
InventorsEugeno Benvenuto, Rosella Franconi, Angiola Desiderio, Paraskevi Tavladoraki
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Abstract
Object of the present invention are peptides which stabilize antibodies including them even in a reducing medium, in particular cytoplasmic medium, the polypeptides which include at least one of the above mentioned peptides, antibodies which include these polypeptides, and polynucleotides coding for peptides, polypeptides and antibodies of the invention.
Claims33
A peptide characterized
- in comprising a sequence selected from the group conisting of the sequences reported in the annexed sequence listing from SEQ ID NO:
- 1 to SEQ ID N:
- 8,
- and in that
- included in a variable region of an antibody make said antibody soluble and stable in cytoplasm,
- the peptides comprising the sequences from SEQ ID NO:
- 1 to SEQ ID NO:
- 4 being included in the variable region of the heavy chain of an antibody, covalently linked to peptides comprising the sequences reported in the annexed sequence listing from SEQ ID NO:
- 88 to SEQ ID NO:
- 90
- in the order
- SEQ ID NO:1-SEQ ID NO:88-SEQ ID NO:2-SEQ ID NO:89-SEQ ID NO:3-SEQ ID NO:90-SEQ ID NO:4, and
- the peptides comprising the sequences from SEQ ID NO:
- 5 to SEQ ID NO:
- 8 being included in the variable region of the light chain of an antibody, covalently linked to peptides comprising the sequences reported in the annexed sequence listing from SEQ ID NO:
- 91 to SEQ ID NO:
- 93
- in the order
- SEQ ID NO:5-SEQ ID NO:91-SEQ ID NO:6-SEQ ID NO:92SEQ ID NO:7-SEQ ID NO:93-SEQ ID NO:8:
A process for preparing a polypeptide suitable as a variable region of an antibody stable and soluble in cytoplasm and specific for a predetermined antigen comprising the step of:
- producing an antibody having as a variable region of the heavy chain the polypeptide comprising the sequence reported in the sequence listing as SEQ ID NO:
- 101, and as a variable region of the light chain the polypeptide comprising the sequence reported in the annexed sequence listing as SEQ ID NO:
- 102;
- putting in contact said antibody with said antigen and
- selecting the antibody binding said antigen,
- isolating the polypeptide of the variable region of the heavy chain and/or the polypeptide of the variable region of the light chain of the antibody binding said antigen.
A polypeptide comprising the sequence reported in the annexed sequence listing as SEQ ID NO:
- 101 or SEQ ID NO:
- 102.
A process for producing a peptide conferring to an antibody binding specificity to a predetermined antigen comprising the step of:
- producing an antibody having as a variable region of the heavy chain the polypeptide comprising the sequence reported in the sequence listing as SEQ ID NO:
- 101, and as a variable region of the light chain the polypeptide comprising the sequence reported in the annexed sequence listing as SEQ ID NO:
- 102;
- putting in contact said antibody with said antigen and
- selecting the antibody binding said antigen,
- isolating the polypeptide of the variable region of the heavy chain and/or the polypeptide of the variable region of the light chain of the antibody binding said antigen;
- isolating from said polypeptide the part conferring binding specificity to said antibody.
A polynucleotide according to any one of the claims 25 to 29, for use as a medicament.
Dependencies are inferred from the claim text.