Identification of a pluripotent pre-mesenchymal, pre-hematopoietic progenitor cell
AssigneeUniversity of Southern California, a California corporation
InventorsFrederick L. Hall, Erlinda M. Gordon
Life of this patent
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Abstract
The present invention provides a molecular marker for the identification of pluripotent pre-mesenchymal, pre-hematopoietic stem cells. The invention further provides primitive progenitor cells identified by the molecular marker. Such cells have the potential to differentiate into both mesenchymal and hematopoietic phenotypes, as determined by a proliferative response to inductive growth factors and cytokines, and by their morphologic and cytochemical features.
Claims52
An isolated pluripotent pre-mesenchymal, pre-hematopoietic progenitor stem cell.
An isolated pluripotent pre-mesenchymal, pre-hematopoietic stem cell capable of maintaining an undifferentiated state when cultured under conditions which do not induce differentiation or cell death.
A method for ameliorating a connective tissue-related disorder in a subject, comprising administering to the subject a therapeutically effective amount of an isolated, pluripotent pre-mesenchymal, pre-hematopoietic stem cell in a pharmaceutically acceptable carrier.
A method for ameliorating a blood tissue-related disorder in a subject, comprising administering to the subject a therapeutically effective amount of a pluripotent pre-mesenchymal, pre-hematopoietic stem cell in a pharmaceutically acceptable carrier.
A method for promoting bone marrow tissue regeneration in a subject, comprising administering to the subject a therapeutically effective amount of a pluripotent pre-mesenchymal, pre-hematopoietic stem cell, wherein the cell promotes bone marrow regeneration in the subject.
A method for identifying a pre-mesenchymal, pre-hematopoietic stem cell from a population of cells comprising:
- (a) obtaining a population of cells from an animal species;
- (b) culturing the cells of (a) in vitro;
- (c) contacting the cells of (b) with a cell proliferation-modulating agent that induces osteoblast specific factor 2 (Osf2) expression;
- and
- d) identifying a pre-mesenchymal, pre-hematopoietic stem cell that expresses osteoblast specific factor 2 (Osf2).
A method for identifying a pre-mesenchymal, pre-hematopoietic stem cell from a population of cells comprising:
- (a) obtaining a population of cells from an animal species;
- (b) culturing the cells of (a) in vitro;
- (c) contacting the cells of (b) with a cell proliferation-modulating agent that induces the expression of osteoblast specific factor 2 (Osf2);
- (d) introducing a genetic construct comprising a nucleic acid sequence encoding a detectable marker operably associated with an Osf2 regulatory region;
- and
- (e) identifying the cells which express the detectable marker,
- wherein the expression of the detectable marker is indicative of a pre-mesenchymal, pre-hematopoietic stem cell.
A kit useful for the detection of a pre-mesenchymal, pre-hematopoietic stem cell comprising carrier means being compartmentalized to receive in close confinement therein two or more containers comprising:
- (a) a first container containing a cell proliferation modulating agent;
- and
- (b) a second container containing an Osf2-specific probe.
A method for generating a pre-mesenchymal, pre-hematopoietic stem cell from a progenitor stem cell in a sample, comprising contacting the sample with an Osf2 protein.
A method for generating a pre-mesenchymal, pre-hematopoietic stem cell from a progenitor stem cell in a sample, comprising contacting the sample with a nucleic acid encoding Osf2.
Dependencies are inferred from the claim text.