Compositions comprising viruses and methods for concentrating virus preparations
OwnerAndreas Frei, Henry K.H. Kwan +7inventors
InventorsAndreas Frei, Henry K.H. Kwan, Varda E. Sandweiss, Gary J. Vellekamp, Pui-Ho Yuen, Laureano L. Bondoc JR., Frederick William Porter IV, John Chu-Tay Tang, Peter Ihnat
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Abstract
A composition is disclosed comprising virus in a formulation comprising a polyhydroxy hydrocarbon buffered to maintain a pH in a range from about 7 to about 8.5 at a temperature in the range from about 2° C. to 27° C. Methods for concentrating and purifying virus preparations are also disclosed.
Claims28
A composition comprising virus in a formulation comprising a polyhydroxy hydrocarbon buffered to maintain a pH in a range from about 7 to about 8.5 at a temperature in the range from about 2° C. to 27° C.
A method for concentrating a virus preparation comprising:
- (a) adding a polyhydroxy hydrocarbon to a virus preparation to a final polyhydroxy hydrocarbon concentration of about 20% or more;
- and
- (b) subjecting the virus preparation to a filtration process wherein the concentration of virus is increased by applying pressure to the preparation such that diluent is removed from the virus preparation through a filter while the virus is retained.
A method of purifying a virus preparation comprising:
- (a) subjecting the virus preparation to anion-exchange chromatography, wherein the virus is eluted as a virus preparation product from an anion-exchange chromatographic medium;
- (b) adding a polyhydroxy hydrocarbon to the virus preparation product of step (a) so that the concentration of polyhydroxy hydrocarbon in the preparation reaches a level of about 25% or more;
- and
- (c) increasing the concentration of virus in the virus preparation product of step (b) by applying pressure to the preparation such that diluent is removed from the virus preparation through a filter while the virus is retained;
- and
- (d) subjecting the concentrated virus preparation product of step (c) to one or more additional processing steps.
A method for concentrating a virus preparation comprising:
- (a) centrifuging a composition which comprises a first layer comprising a polyhydroxy hydrocarbon in a concentration of 35% to 80% (v/v), the first layer overlaid with a second layer comprising a polyhydroxy hydrocarbon in a concentration of 5% to 30% (v/v), the second layer overlaid with a third layer comprising virus;
- and
- (b) recovering the virus from the first layer.
Dependencies are inferred from the claim text.