Production of tcr gamma delta t cells
OwnerDavid N Bell, Danna L Skeainventors
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Abstract
The method for obtaining and expanding TcRγδ+ T cells in culture is described. The method involves: (1) culturing cells from a sample containing TcRγδ+ T cells or precursors thereof in a first culture medium comprising a T cell mitogen and at least two growth factors and (2) culturing the cells obtained in step (1) in a second culture medium comprising at least two growth factors. The two growth factors are factors with interleukin-2-like and interleukin-7-like activity. The TcRγδ+ T cells obtained by the method can be used in a variety of experimental, therapeutic and commercial applications.
Claims44
A method for expanding TcRγδ+ T cells in a starting sample comprising:
- (1) culturing cells in the starting sample in a first culture medium comprising a T cell mitogen, a growth factor having interleukin-2-like activity and a growth factor having interleukin-7-like activity;
- and
- (2) culturing the cells obtained in step (1) in a second culture medium comprising a growth factor having interleukin-2-like activity and a growth factor having interleukin-7-like activity to expand TcRγδ+ T cells.
A cell preparation enriched in TcRγδ+ T cells wherein greater than 70% of the total cells are TcRγδ+ T cells.
Dependencies are inferred from the claim text.