Methods for the induction of professional and cytokine-producing regulatory T cells

A method for generating regulatory T cells comprising:

• a) treating conventional T cells with a regulatory composition comprising (i) at least one cytokine;
• (ii) at least one T cell activator;
• and (iii) at least one population of non T accessory T cells for a period of time sufficient generate a population of cytokine producing regulatory T cells.

A method for inducing the expression of CD122 on the surface of regulatory T cells comprising:

• treating a population of CD4+ T cells comprising conventional T cells and professional regulatory T cells with a regulatory composition comprising (i) at least one cytokine;
• (ii) at least one T cell activator;
• and (iii) at least one population of non T accessory cells for a period of time sufficient to generate regulatory T cells that express the CD122 marker on their surface.

A method for generating regulatory T cells comprising:

• a) making a CD4+ T cell population comprising activated professional regulatory T cells and a conventional T cells;
• and,
• b) adding at least one T cell activator to said CD4+ T cell population for a period of time sufficient to generate a regulatory cell population comprising professional regulatory T cells and cytokine producing regulatory T cells.