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US 2003/0156990 A1Published application
Specimen slides and methods and uses thereof
AssigneeCarl Zeiss Jena GmbH
InventorsPeter Westphal, Tobias Neumann, Martin Kuehner, Dieter Graefe
Life of this patent
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Abstract
Emboidments are presented to improve spatial resolution or sensitivity in measuring fluorescent layers, including an embodiment wherein a specimen slide is provided which is not fluorescent by itself, is plate-shaped and has at least one plate side provided with fluorescent light absorbing properties, or with an antireflection-coating for fluorescent radiation.
Claims17
A specimen slide comprising two parallel surfaces connected by a thickness to form a plate, wherein the plate is not autofluorescent and
- at least one of the surfaces absorbs fluorescent light, or at least one of the surfaces is at least partially coated with an anti-reflection coating for fluorescent radiation, or at least one of the surfaces is both absorbent of fluorescent light and is coated with an anti-reflection coating.
A method of using a specimen slide comprising:
- providing a specimen slide comprising a first surface parallel to a second surface, with the surfaces being connected by a thickness to form a plate, wherein fluorescent radiation incident on the first surface is not reflected by the plate back to the first surface,
- and using the specimen slide for fluorescence microscopy in a non-confocal fluorescence microscope.
A fluorescence microscope comprising:
- a microscope, a source for fluorescence exciting radiation, and a specimen slide support that comprises a top surface for receiving a specimen slide wherein fluorescent radiation incident on the top surface is not back-reflected by the specimen slide support to the top surface.
Dependencies are inferred from the claim text.