Fluidic device for medical diagnostics

A fluidic diagnostic device for measuring an analyte concentration or property of a biological fluid, comprising

• a first layer, which has a resilient region over at least a part of its area, and a second layer, separated by an intermediate layer, in which recesses in a first surface of the intermediate layer form, with the first layer,
• a) a sample port for introducing a sample of the biological fluid into the device;
• b) a measurement area, in which the sample undergoes a change in a physical property that is measured and related to the analyte concentration or property of the fluid;
• c) a channel, having a first end and a second end, to provide a fluidic path from the sample port at the first end through the measurement area;
• and
• d) a bladder, at the second end of the channel, comprising the resilient region in the first layer and having a volume that is at least about equal to the combined volume of the measurement area and channel;
• and
• a stop junction in the channel between the measurement area and bladder that comprises two passages substantially normal to the first surface of the intermediate layer, each passage having a first end in fluid communication with the channel and a second end in fluid communication with a recess in a second surface of the intermediate layer, which recess provides fluid communication between the second ends of the passages.