Method and arrangement for reducing noise
AssigneeGyros AB
InventorTobias Soderman
Life of this patent
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Abstract
The present invention concerns a method and an arrangement for reducing noise in a substance raw data image containing noise. The method may comprise following steps in any order for removing different noise signals contributions step-by-step in any order in a substance raw data image: reducing background radiation; reducing peak disturbance; locating the detection area within the search area; moving binary artifacts; removing unwanted areas of the detection area; and applying default detection area in noisy images). The present invention also relates to a method and an arrangement for determining a measure of at least one substance.
Claims38
A method for reducing noise in a substance raw data image having substance and noise data which is associated to one detection microcavity and one substance is present in the detection microcavity of a microchannel structure of a microfluidic device, the substance raw data information obtained by use of a detector arrangement capable of scanning and collecting radiation data in a search area associated to a detection area, the collected radiation data are stored as measure data for the amount of the substance as substance raw data in a set in a data storage, said method comprises the step of:
- reducing background radiation data either by use of a image comprising background radiation data and no substance data information, a background radiation image, or by use of a calculated median value of the background radiation data.
A method for determining a measure of at least one substance comprising the steps of:
- (a) providing a microfluidic device and a detector arrangement, wherein said microfluidic device comprises a plurality of microchannel structures, each of which has an inlet port and a detection microcavity, and a plurality of detection areas, each of which is associated with one of said detection microcavities;
- and wherein said detector arrangement is capable of collecting radiation from individual subareas of each of said detection areas;
- (b) processing one or more liquid aliquots in at least one of said plurality of microchannel structures so that said substance is retained in the detection microcavity of each of said at least one of said plurality of microchannel structures;
- (c) scanning the detection areas associated with the detection microcavities that are part of microchannel structures in which step (b) has been carried out to obtain radiation from individual subareas (pixels) of each scanned detection area, said scanning being performed by the use of said detector arrangement;
- (d) integrating radiation as a function of subarea for each scanned detection area to obtain the amount of radiation from each detection area;
- and
- (e) characterizing for each of the amounts obtained in step (d) a reaction variable that has been included in the process protocol used for each microchannel structure.
An arrangement for reducing noise in a substance raw data image of having substance and noise data which is associated to one detection microcavity and one substance retained in the detection microcavity of a microfluidic structure of a microfluidic device, the substance raw data information obtained by use of a detector arrangement capable of scanning and collecting radiation data in a search area associated to a detection area, which is associated to the detection microcavity, the collected radiation data are stored as measure data for the amount of the substance as substance raw data in a set in a data storage, the arrangement further comprises a processing unit for controlling the arrangement, the arrangement comprises:
- means for reducing background radiation data either by use of an image comprising background radiation data and no substance data information or by use of a calculated median value of the background radiation data.
An arrangement for determining a measure of at least one substance, the arrangement comprises:
- (a) a microfluidic device and a detector arrangement, wherein said microfluidic device comprises a plurality of microchannel structures, each of which has an inlet port and a detection microcavity, and a plurality of detection areas, each of which is associated with one of said detection microcavities;
- and wherein said detector arrangement is capable of collecting radiation from individual subareas of each of said detection areas;
- (b) means for integrating radiation as a function of subarea for each scanned detection area to obtain the amount of radiation from each detection area;
- and
- (c) means for characterizing for each of the amounts obtained in step (d) a reaction variable that has been included in the process protocol used for each microchannel structure.
Dependencies are inferred from the claim text.