Triacylglycerol lipases
OwnerMartina Korner, Ekkehart Berndt +2inventors
InventorsMartina Korner, Ekkehart Berndt, Kathrin Fritsche, Ivo Feussner
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Abstract
The invention relates to nucleic acid molecules, which code for proteins with the enzymatic activity of an acyl hydrolase, in particular, a triacylglyercol lipase (TAG-lipase) and method for the production of transgenic plants, which contain said nucleic acid molecules and whose acyl hydrolase content, in particular of TAG-lipase is altered in comparison to wild type plants. The invention further relates to said novel plants, the parts, products and plant cells thereof and the use of the nucleic acid molecules to influence the content of poylunsaturated fatty acids in transgenic plants.
Claims25
A method of producing plants or plant cells, respectively, which have an altered content of unsaturated fatty acids and particularly of oxygenated polyene fatty acids, comprising the following steps,
- a) producing a nucleic acid sequence comprising the following components, which are successively arranged in 5′-3′-orientation:
- a promoter that is functional in plants and particularly seed-specific,
- at least one DNA sequence that codes for a protein having the enzymatic activity of a TAG lipase, more preferably of a TAG lipase which is specific for oxygenated polyene fatty acids, selected from the group consisting of:
- i. DNA sequences comprising the nucleotide sequence identified in SEQ ID NO:
- 1,
- ii. DNA sequences comprising the nucleotide sequence identified in SEQ ID NO:
- 3,
- iii. DNA sequences comprising the nucleotide sequence identified in SEQ ID NO:
- 5,
- iv. DNA sequences that encode the amino acid sequence identified in SEQ ID NO:
- 2 or fragments thereof,
- v. DNA sequences that encode the amino acid sequence identified in SEQ ID NO:
- 4 or fragments thereof,
- vi. DNA sequences that encode the amino acid sequence identified in SEQ ID NO:
- 6 or fragments thereof,
- vii. DNA sequences comprising a nucleotide sequence having a sequence identity of at least 60%, preferably of more than 80% and more preferably of more than 90% to the sequences mentioned in i. to vi.,
- viii. DNA sequences comprising one of the sequences mentioned in i. to vii. in anti-sense orientation,
- b) transferring the nucleic acid from a) to plant cells and, optionally, integrating the nucleic acid sequence into the plant genome.
Use of a nucleic acid sequence, selected from the group consisting of:
- i. DNA sequences comprising the nucleotide sequence identified in SEQ ID NO:
- 1,
- ii. DNA sequences comprising the nucleotide sequence identified in SEQ ID NO:
- 3,
- iii. DNA sequences comprising the nucleotide sequence identified in SEQ ID NO:
- 5,
- iv. DNA sequences encoding the amino acid sequence identified in SEQ ID NO:
- 2 or fragments thereof,
- v. DNA sequences encoding the amino acid sequence identified in SEQ ID NO:
- 4 or fragments thereof,
- vi. DNA sequences encoding the amino acid sequence identified in SEQ ID NO:
- 6 or fragments thereof,
- vii. DNA sequences comprising a nucleotide sequence that has a sequence identity of at least 60%, preferably of more than 80% and more preferably of more than 90% to the sequences mentioned in i. to vi.,
- viii. DNA sequences comprising one of the sequences mentioned in i. to vii. in anti-sense orientation,
- for producing transgenic plants or plant cells, respectively, having an altered content of unsaturated fatty acids, particularly of oxygenated polyene fatty acids, particularly in the seed oil.
A method of altering the content of unsaturated fatty acid residues, especially of oxygenated polyene fatty acid residues, in transgenic plants by influencing the lipoxygenase(LOX)-dependent metabolism of poly-unsaturated fatty acids in transgenic plants.
Use of a TAG lipase, especially of a TAG lipase that is specific for oxygenated polyene fatty acids and that is encoded by a nucleic acid molecule, selected from the group consisting of:
- i. DNA sequences comprising the nucleotide sequence identified in SEQ ID NO:
- 1
- ii. DNA sequences comprising the nucleotide sequence identified in SEQ ID NO:
- 3,
- iii. DNA sequences comprising the nucleotide sequence identified in SEQ ID NO:
- 5,
- iv. DNA sequences that encode the amino acid sequence identified in SEQ ID NO:
- 2 or fragments thereof,
- v. DNA sequences that encode the amino acid sequence identified in SEQ ID NO:
- 4 or fragments thereof,
- vi. DNA sequences that encode the amino acid sequence identified in SEQ ID NO:
- 6 or fragments thereof,
- vii. DNA sequences comprising a nucleotide sequence that has a sequence identity of at least 60%, preferably of more than 80% and more preferably of more than 90% to the sequences mentioned in i. to vi.,
- for processing plant oils, which comprise oxygenated polyene fatty acids.
Dependencies are inferred from the claim text.