Transgenic non-human mammal with an oncogenic mutant of the c-Raf-1 gene

A transgenic mouse, which is obtainable by the following steps:

• a) integration of the cDNA sequence of a constitutively active oncogenic mutant of the kinase domain of the c-Raf-1 gene or of a corresponding normal allele of the Raf gene or a derivative of the A Raf gene, the B Raf gene or the c-Raf-1 gene in an expression vector to provide an expression vector carrying a foreign DNA,
• b) insertion of the transgenic vector obtained in step a) in pronuclei of fertilized oocytes from a mouse,
• c) implantation of the oocytes obtained in step b) in brood animals of the same species as the donor species of the oocytes and delivery of descendant animals from the oocytes,
• d) genotypization and selection of the descendant animals obtained in step c) with the proviso that cells of the selected mice express a constitutively active oncogenic mutant of the kinase domain of the c-Raf-1 gene or a protein coded by a corresponding normal allele of the Raf gene or a derivative of the A Raf gene, the B Raf gene or the c-Raf-1 gene.

A method for producing a transgenic mouse, including the following steps:

• a) integration of the cDNA sequence of a constitutively active oncogenic mutant of the kinase domain of the c-Raf-1 gene or of a corresponding normal allele of the Raf gene or a derivative of the A Raf gene, the B Raf gene or the c-Raf-1 gene in an expression vector to provide an expression vector carrying a foreign DNA,
• b) insertion of the transgenic vector obtained in step a) in pronuclei of fertilized oocytes from a mouse,
• c) implantation of the oocytes obtained in step b) in brood animals of the same species as the donor species of the oocytes and delivery of descendant animals from the oocytes,
• d) genotypization and selection of the descendant animals obtained in step c) with the proviso that cells of the selected mice express a constitutively active oncogenic mutant of the kinase domain of the c-Raf-1 gene or a protein coded by a corresponding normal allele of the Raf gene or a derivative of the A Raf gene, the B Raf gene or the c-Raf-1 gene.

A method for producing cell tissue from a transgenic mouse, including the following steps:

• a) integration of the cDNA sequence of a constitutively active oncogenic mutant of the kinase domain of the c-Raf-1 gene or of a corresponding normal allele of the Raf gene or a derivative of the A Raf gene, the B Raf gene or the c-Raf-1 gene in an expression vector to provide an expression vector carrying a foreign DNA,
• b) insertion of the transgenic vector obtained in step a) in pronuclei of fertilized oocytes from a mouse,
• c) implantation of the oocytes obtained in step b) in brood animals of the same species as the donor species of the oocytes and delivery of descendant animals from the oocytes,
• d) genotypization and selection of the descendant animals obtained in step c) with the proviso that cells of the selected mice express a constitutively active oncogenic mutant of the kinase domain of the c-Raf-1 gene or a protein coded by a corresponding normal allele of the Raf gene or a derivative of the A Raf gene, the B Raf gene or the c-Raf-1 gene,
• e) removal of cell tissue from the mouse.