Universal markers of transgenesis
OwnerErnst A. Wimmer, Andreas J. Berghammer +1inventors
InventorsErnst A. Wimmer, Andreas J. Berghammer, Martin Klingler
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Abstract
The invention relates to methods, cells and nucleic acids for making transgenic animals. The methods generally comprise introducing into a genome of an animal a genetic construct comprising a transcriptional regulatory element operably linked to a heterologous marker gene encoding a marker, wherein the element drives expression of the marker across genera transgenic in the construct sufficient to visually detect the marker in photoreceptive cells or organs, and selecting for transgenesis by visually detecting the marker in a photoreceptive cell or organ of the animal.
Claims25
A method of making a transgenic animal comprising the steps of:
- (a) introducing into a genome of an animal a genetic construct comprising a transcriptional regulatory element operably linked to a heterologous marker gene encoding a marker, wherein the element drives expression of the marker across genera transgenic in the construct sufficient to visually detect the marker in photoreceptive cells or organs, and (b) selecting for transgenesis by visually detecting the marker in a photoreceptive cell or organ of the animal.
A polytropic vector functional in nondipteran species and comprising a transcriptional regulatory element operably linked to a heterologous marker gene encoding a marker, wherein the element drives expression of the marker across genera transgenic in the construct sufficient to visually detect the marker in photoreceptive cells or organs.
Dependencies are inferred from the claim text.